FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763918
Sequences flagged as poor quality0
Sequence length32-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG15210.19910513955686346Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG13390.17528059294322165Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGGCGGGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG8830.11558832230684445No Hit
GATCGGGAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG8020.10498509002275114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)