FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001303675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001303675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922585
Sequences flagged as poor quality0
Sequence length138-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT229982.4927784431786773TruSeq Adapter, Index 19 (97% over 38bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT44920.48689280662486384TruSeq Adapter, Index 12 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)