FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001303691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001303691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923403
Sequences flagged as poor quality0
Sequence length140-151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT402454.358335418013587TruSeq Adapter, Index 19 (97% over 38bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT78870.854123280951004TruSeq Adapter, Index 12 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)