FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001303699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001303699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348930
Sequences flagged as poor quality0
Sequence length138-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT337822.5043553038334085TruSeq Adapter, Index 19 (97% over 38bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT67910.5034360567264424TruSeq Adapter, Index 12 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)