FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001303709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001303709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299345
Sequences flagged as poor quality0
Sequence length137-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT134381.0342133921321897TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTAT60130.46277162724295706TruSeq Adapter, Index 3 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT15730.12106099611727447TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTGGTAT15600.1200604920171317TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)