FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001303779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001303779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504099
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1395355.572263716410573TruSeq Adapter, Index 27 (97% over 39bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA37100.1481570816489284No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA34350.13717508772616419No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29880.11932435578625286No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)