FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001303801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001303801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589875
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT307045.2051705869887686TruSeq Adapter, Index 27 (97% over 39bp)
CTGAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAA10390.17613901250264885No Hit
ATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTG7780.13189235007416825No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA6760.11460055096418732No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA5970.10120788302606484No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)