FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001303871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001303871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547731
Sequences flagged as poor quality0
Sequence length139-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT212413.877998506566179TruSeq Adapter, Index 7 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT29020.5298221207125395TruSeq Adapter, Index 2 (97% over 35bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA5700.10406568187668763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)