FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001303895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001303895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749979
Sequences flagged as poor quality0
Sequence length137-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT118221.576310803369161TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTAT41260.5501487374979833TruSeq Adapter, Index 6 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT12250.1633379067947236TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTGGTAT10870.1449373915802976TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGGAT9990.13320372970443173TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGTAT9230.1230701126298203TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGGAT8190.1092030576856152TruSeq Adapter, Index 6 (97% over 36bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA7540.100536148345487No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)