FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001303899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001303899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466289
Sequences flagged as poor quality0
Sequence length137-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT269531.838177876257682TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTAT57260.3905096471432303TruSeq Adapter, Index 6 (97% over 36bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA34750.23699284383910676No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA24730.16865706555801754No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)