FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001303901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001303901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566805
Sequences flagged as poor quality0
Sequence length138-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT278581.7780132179818164TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTAT36260.23142637405420585TruSeq Adapter, Index 6 (97% over 36bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA35380.2258098487048484No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT29490.18821742335517183TruSeq Adapter, Index 3 (97% over 34bp)
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA24930.1591136101812287No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)