FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001303904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001303904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1203424
Sequences flagged as poor quality0
Sequence length138-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243392.022479192703486No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA59540.4947549658308294No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA46670.3878101151381392No Hit
AGTGGGATTCCTGCTGTCAGTTAAAAAGTCAGGAAACAACAGGTGCTGGA26780.22253170952216342No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTTGGA23320.19378041322094292No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTAAAGAGCTTCTGCACAGCAAAAG23050.1915368149546627No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT18070.15015489137660543No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA16840.13993405483021779No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCCATTCCAT16230.13486518467306619No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA16120.1339511261201372No Hit
AGTGGGATTCCTGCTGTCAGTTAATCCAGTCTATCATTGTTGGACATTTG14040.11666710984657112No Hit
AGTGGGATTCCTGCTGTCAGTTAAGAAAATGTGGCACATATACACCATGG12590.10461815619432552No Hit
AGTGGGATTCCTGCTGTCAGTTAATGATTGCCATTCTAACTGGTGTGAGA12250.10179288430345415No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTGTTCATAGAGCAGTTAGGA12120.10071263328635625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)