FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001303917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001303917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849900
Sequences flagged as poor quality0
Sequence length53-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT196582.31297799741146TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTAT24450.28768090363572185TruSeq Adapter, Index 6 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT21670.25497117307918576TruSeq Adapter, Index 3 (97% over 34bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA17460.20543593363925167No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA13160.15484174608777504No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)