FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001303922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001303922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760043
Sequences flagged as poor quality0
Sequence length138-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG222531.2643441097745907No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA53400.30340167825445175No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA44070.2503916097504436No Hit
AGTGGGATTCCTGCTGTCAGTTAAAAAGTCAGGAAACAACAGGTGCTGGA43410.2466417013675234No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTAAAGAGCTTCTGCACAGCAAAAG32800.1863590832723973No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA27640.1570416177332031No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA26250.14914408341159846No Hit
AGTGGGATTCCTGCTGTCAGTTAATGATTGCCATTCTAACTGGTGTGAGA26110.14834864830006994No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTTGGA24990.14198516740784173No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT23260.1321558621010964No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCCATTCCAT22230.12630373235199369No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)