FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001303923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001303923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701738
Sequences flagged as poor quality0
Sequence length137-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT239751.4088537718497207TruSeq Adapter, Index 2 (97% over 37bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA23110.13580233855035262No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA17930.10536287019505941No Hit
AGTGGGATTCCTGCTGTCAGTTAAAAAGTCAGGAAACAACAGGTGCTGGA17390.10218964376419871No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)