FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001317900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001317900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854700
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50790.5942435942435942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49270.5764595764595765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31970.37404937404937405No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT31020.36293436293436293No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24610.28793728793728796No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT22270.26055926055926054RNA PCR Primer, Index 46 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14400.16848016848016847No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT11840.13852813852813853RNA PCR Primer, Index 46 (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11610.13583713583713583Clontech SMART CDS Primer II A (100% over 21bp)
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG9020.10553410553410553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)