FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001317907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001317907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913912
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80570.42097024314597536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68100.35581573238477004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48580.25382567223571406No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40650.21239221030016012No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT34800.18182654165917764No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG28320.1479691856260894No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG27900.14577472736468552No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC27790.1451999882962226No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA25580.13365295792074036No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA24320.12706958313652875No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT23880.12477062686267706RNA PCR Primer, Index 46 (95% over 21bp)
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT23640.12351665071330344No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA22640.11829175009091326No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA22450.11729901897265915No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21520.11243986139383629No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG21180.11066339518222364No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC21030.10987966008886511No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT19610.10246030120507107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)