FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001317914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001317914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114504
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT22271.944910221476979No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT19281.6837839726123107TruSeq Adapter, Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14561.2715712988192553No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11280.9851184238105218No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT9440.8244253475861105RNA PCR Primer, Index 11 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8630.7536854607699295No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG7850.685565569761755TruSeq Adapter, Index 11 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6930.6052190316495494No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC4350.3798993921609725No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT4240.37029274086494796TruSeq Adapter, Index 11 (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.31614616083280933No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.30741284147278697No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT2990.26112624886466845No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2790.24365961014462378Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG2650.2314329630405925TruSeq Adapter, Index 11 (95% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.21309299238454552Clontech SMART CDS Primer II A (100% over 21bp)
CNTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT2320.20261300915251867No Hit
ANACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT2030.17728638300845387TruSeq Adapter, Index 11 (95% over 21bp)
CAGGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.151959756864389No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1710.1493397610563823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1630.14235310556836445No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC1570.137113113952351RNA PCR Primer, Index 17 (95% over 24bp)
CAGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.1344931181443443No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1390.12139313910431078No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1330.11615314748829736Clontech Universal Primer Mix Long (96% over 25bp)
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA1250.10916649200027946RNA PCR Primer, Index 11 (96% over 28bp)
GATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT1240.10829316006427724No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA1240.10829316006427724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1210.10567316425627053No Hit
ANAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.10567316425627053No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACTA1200.10479983232026828No Hit
CAGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.10392650038426605No Hit
TNTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG1160.10130650457625934TruSeq Adapter, Index 11 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)