FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001317915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001317915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117541
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT25002.1269174160505697No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT24112.051199156039169TruSeq Adapter, Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15211.2940165559251666No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13081.112803192077658No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT11080.9426497987936124RNA PCR Primer, Index 11 (95% over 23bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG10660.9069175862039629TruSeq Adapter, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9170.7801533082073489No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.6210598854867663No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC5340.4543095600684017No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT5180.44069728860567803TruSeq Adapter, Index 11 (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.3454113883666125No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.30882840881054274No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT3620.30797764184412246No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG3020.2569316238589088TruSeq Adapter, Index 11 (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2880.24502088632902563Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.20503483890727492Clontech SMART CDS Primer II A (100% over 21bp)
CAGGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.18801949957887035No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.17781029598182763No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA1870.1590934227205826RNA PCR Primer, Index 11 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1730.14718268519069944No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA1730.14718268519069944No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC1660.1412273164257578RNA PCR Primer, Index 17 (95% over 24bp)
CAGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.1378242485600769No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1610.1369734815936567No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1470.1250627440637735No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACTA1410.11995814226525212No Hit
GCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG1340.11400277350031053No Hit
CAGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.11059970563462962No Hit
GATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT1270.10804740473536895No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1190.10124126900400712Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC1180.10039050203758687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)