FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001318032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001318032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54907
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT610611.120622142896169TruSeq Adapter, Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT31055.655016664541862No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT30675.5858087311271785RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT10591.9287158285828765TruSeq Adapter, Index 1 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC8391.528038319339975No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT8321.5152894895004279No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCAT6861.2493853242755932No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG6771.2329939716247473TruSeq Adapter, Index 1 (95% over 24bp)
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC5230.9525197151547161Illumina PCR Primer Index 5 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3830.6975431183637788No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3570.6501903218168904No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG2480.45167282860108915TruSeq Adapter, Index 1 (96% over 28bp)
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC2170.395213725025953No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.3715373267525088No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1770.32236326879997085Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.31325696177172313No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.24222776695139053No Hit
CTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC980.17848361775365618No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.16027100369716066No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTAGGCAT880.16027100369716066No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.15116469666891288Clontech SMART CDS Primer II A (100% over 21bp)
TTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT800.14570091245196423No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT800.14570091245196423Clontech Universal Primer Mix Long (96% over 25bp)
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTT790.14387965104631467TruSeq Adapter, Index 1 (96% over 27bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT730.13295208261241737Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC630.11473946855592182Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT630.11473946855592182TruSeq Adapter, Index 1 (95% over 21bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC600.10927568433897318Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG600.10927568433897318Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC560.10199063871637495Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA550.10016937731072541Clontech Universal Primer Mix Long (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)