FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001318034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001318034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949330
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT214142.25569612252852No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT181481.9116640156742124RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT116891.2312894357072883RNA PCR Primer, Index 39 (96% over 25bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG110621.1652428554875545RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT54890.5781972549060916RNA PCR Primer, Index 39 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50090.5276352796182571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46780.4927685841593545No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG34990.3685757323586108Illumina PCR Primer Index 11 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33030.3479295924494117No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC27140.28588583527329803No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24810.26134220976899497No Hit
CNTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT24450.2575500616224074No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA21360.2250007900308639RNA PCR Primer, Index 39 (96% over 30bp)
ANACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT21230.2236314032001517RNA PCR Primer, Index 39 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT20530.21625778180400915RNA PCR Primer, Index 39 (95% over 22bp)
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC16530.17412280239748032Illumina PCR Primer Index 11 (96% over 26bp)
ANACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT13460.14178420570296946Illumina PCR Primer Index 11 (95% over 24bp)
TNTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG12900.13588530858605544RNA PCR Primer, Index 39 (96% over 30bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11850.12482487649184161Clontech SMART CDS Primer II A (100% over 21bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCTCTCTA10120.1066014978985179No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC10090.10628548555296895RNA PCR Primer, Index 39 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9860.10386272423709353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)