FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001318872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001318872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741478
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21907812.580003881760208Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1213266.9668408099327115Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT901565.176981851048362Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC895575.142585780584079Clontech Universal Primer Mix Long (96% over 25bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT566543.253213649555148Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT556693.1966524986247316Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC450652.587744433176876Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC426742.450447263761012Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC400132.2976460225165063Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG363982.0900637274774647Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA330291.8966073645489638Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA315321.8106458996323813Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC281181.61460552473244Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC260091.4935014970042688Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA219751.2618591793867049Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA219381.2597345473212984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA213871.2280947562932176Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT210941.2112699672347282Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC200181.1494833698731766Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG197101.1317972434908738Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC182271.0466396934098507Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG178821.0268289349621413Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT175731.0090853860915843Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT163270.9375369657268138Clontech SMART CDS Primer II A (96% over 26bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG134010.7695187650949366Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC118760.6819494705072358Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT92370.5304115240043228Clontech Universal Primer Mix Long (96% over 25bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT80850.4642608175354498Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG80700.46339948021163635Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA78300.44961808303062106Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA75860.435606995896589Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG69110.39684681632498375Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA68130.39121941247606923Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA67690.3886928229928831Clontech Universal Primer Mix Long (96% over 25bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA65020.3733610186290036Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT61650.3540096400873281Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA60050.3448220419666513Clontech Universal Primer Mix Long (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC58950.338505568258686Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC58320.3348879514986695Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC55420.3182354299049428Clontech SMART CDS Primer II A (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT55140.31662760023382436Clontech Universal Primer Mix Long (96% over 28bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG53680.3082439169487068Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52960.3041094977944023Clontech Universal Primer Mix Long (100% over 24bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG52570.30187002075248726Clontech SMART CDS Primer II A (96% over 26bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC51820.29756333413342Clontech SMART CDS Primer II A (96% over 26bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA49110.28200183981652366Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG45800.26299499620437355Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45040.25863088709705206Clontech Universal Primer Mix Long (96% over 27bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC35920.20626157780919427Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT33770.1939157428345348Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG33130.19024070358626408Clontech Universal Primer Mix Long (96% over 25bp)
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC31390.18024919063002806Clontech Universal Primer Mix Long (96% over 25bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT30640.17594250401096082Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28850.16566387861345364Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG27780.15951967237025103Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27730.15923255992897986Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT27610.1585434900699291Clontech Universal Primer Mix Long (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT26580.1526289737797434Clontech Universal Primer Mix Long (96% over 28bp)
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG26220.15056176420259113Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA26070.1497004268787777Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24010.1378713942984063Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT22970.13189945551996637Clontech SMART CDS Primer II A (96% over 26bp)
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG22320.1281669937834414Clontech Universal Primer Mix Long (96% over 25bp)
GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG21990.12627205167105182Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA20930.12018526791610346Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGC20280.1164528061795785Clontech Universal Primer Mix Long (96% over 28bp)
GCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGT19850.1139836391846466Clontech SMART CDS Primer II A (95% over 24bp)
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA18980.1089878827065286Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGT18930.10870077026525744Clontech Universal Primer Mix Long (96% over 28bp)
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA18870.10835623533573205Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTTCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC17560.10083388937442792Clontech SMART CDS Primer II A (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)