FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001318885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001318885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508284
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56110.3720121674697869No Hit
GATTTATAGTACAACCAACAAATATTCCTGGACCCCTGCCCGGTTCTCAG49430.3277234260921683No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT48090.31883915761222686No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA43630.28926912968645163No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC43150.28608670515632334No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT43120.28588780362319033No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA40960.27156689323761307No Hit
CTATAAATCAGTGCCCTGGGTACTTGTCCAGGTTTTTGCTGGAACCAGTT40900.27116909017134705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40380.2677214635970414No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC38300.2539309572998189No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC36920.24478148677570008No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC36470.24179796377870483No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC36400.24133386020139444No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA35950.2383503372043992No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT34800.23072577843430017No Hit
ACCTAGGACGGTGAGTCTGGTCCCAGCTCCGAAGACCCGACCATCACCAT34430.22827265952565962No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC33640.22303491915315685No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32920.2182612823579644No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG31370.20798470314609185No Hit
GACCTAGGACGGTGAGTCTGGTCCCAGCTCCGAAGACCCGACCATCACCA30400.20155355357479096No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAG29600.19624951269124383No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA29310.194326797870958No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT29000.19227148202858346No Hit
GACTATTACTGCCTACTCAACTATGGTGATGGTCGGGTCTTCGGAGCTGG28910.19167477742918443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27450.1819949028167109No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG27210.1804036905516468No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26540.17596155631167604No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT25920.17185092462692703No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG24500.16243625205863088No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT23190.15375088511182244No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCA23070.15295527897929037No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22990.15242487489093565No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG22620.1499717559822951No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG22450.14884464729454136No Hit
GCCTTGGGCTGACCTAGGACGGTGAGTCTGGTCCCAGCTCCGAAGACCCG21990.14579482378650174No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG21600.14320910385577254No Hit
GCCTGAGGACGAGGCTGACTATTACTGCCTACTCAACTATGGTGATGGTC21590.14314280334472818No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT20750.1375735604170037No Hit
TTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG20550.13624755019611692No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA20540.13618124968507256No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA20500.1359160476408952No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG20410.13531934304149618No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC19820.13140761288988015No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT19670.13041310522421506No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAG19400.1286229914260179No Hit
CCATAGTTGAGTAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACC19250.12762848376035282No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG18310.12139623572218494No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18260.12106473316696326No Hit
ATCCAAACTGGTTCCAGCAAAAACCTGGACAAGTACCCAGGGCACTGATT18080.11987132396816513No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17650.11702040199325857No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC17580.1165562984159482No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG17430.1155617907502831No Hit
CAACTATGGTGATGGTCGGGTCTTCGGAGCTGGGACCAGACTCACCGTCC17290.11463358359566236No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCC17060.11310867184164257No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG16950.11237936622015482No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG16840.11165006059866708No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16830.11158376008762276No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16640.1103240503777803No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG16630.11025774986673598No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA16370.10853393657958316No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG16330.1082687345354058No Hit
TCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCAC16270.10787093146913977No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG16170.10720792635869637No Hit
GCACTGGAGCAGTCACCAGTGATTACTATCCAAACTGGTTCCAGCAAAAA16000.1060808176709426No Hit
GTCCAGGAATATTTGTTGGTTGTACTATAAATCAGTGCCCTGGGTACTTG15950.10574931511572092No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGAGTCTGGTC15690.10402550182856811No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC15300.10143978189783888No Hit
GTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGCCTGGAGCTGCAG15280.10130718087575019No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG15240.10104197883157283No Hit
ACTATGGTGATGGTCGGGTCTTCGGAGCTGGGACCAGACTCACCGTCCTA15200.10077677678739548No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15200.10077677678739548No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)