FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001318886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001318886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508284
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTTATAGTACAACCAACAAATATTCCTGGACCCCTGCCCGGTTCTCAG55020.3647854117659539No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52590.3486743875821795No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT47730.3164523392146307No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT43600.28907022815331856No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA41250.2734896080578989No Hit
CTATAAATCAGTGCCCTGGGTACTTGTCCAGGTTTTTGCTGGAACCAGTT40770.27030718352777067No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC39560.2622848216914056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37310.2473672067064293No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA37300.24730090619538495No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA36900.24464888575361138No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT35640.23629502136202465No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC34960.23178658661100962No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC34110.22615104317224077No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC32610.2162059665155899No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC32420.21494625680574747No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC31790.21076932460995407No Hit
ACCTAGGACGGTGAGTCTGGTCCCAGCTCCGAAGACCCGACCATCACCAT31250.2071890970135598No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30160.19996234130972682No Hit
GACTATTACTGCCTACTCAACTATGGTGATGGTCGGGTCTTCGGAGCTGG28530.18915535800949954No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG28390.1882271508548788No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA27420.1817960012835779No Hit
GACCTAGGACGGTGAGTCTGGTCCCAGCTCCGAAGACCCGACCATCACCA27200.18033739004060242No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT27200.18033739004060242No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG26990.17894507930867132No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAG26190.17364103842512418No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA25350.1680717954973997No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT25220.1672098888538233No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24890.16502197198936008No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA24360.1615080449040101No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24330.1613091433708771No Hit
TTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG23780.15766261526343844No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23500.15580620095419695No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG23300.15448019073331018No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCA22360.1482479426951423No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT22260.1475849375846989No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG22040.14612632634172343No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21630.14340800538890555No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG21050.13956257574833386No Hit
GCCTTGGGCTGACCTAGGACGGTGAGTCTGGTCCCAGCTCCGAAGACCCG20290.1345237369089641No Hit
GCCTGAGGACGAGGCTGACTATTACTGCCTACTCAACTATGGTGATGGTC20230.13412593384269808No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA19360.12835778938184056No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT19140.1268991781388651No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAG19120.1267665771167764No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19100.12663397609468774No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC18910.1253742663848453No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA18650.12365045309769247No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG18480.12252334440993871No Hit
CCATAGTTGAGTAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACC18340.12159513725531795No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG17860.11841271272518969No Hit
ATCCAAACTGGTTCCAGCAAAAACCTGGACAAGTACCCAGGGCACTGATT17820.11814751068101233No Hit
GCACTGGAGCAGTCACCAGTGATTACTATCCAAACTGGTTCCAGCAAAAA17130.11357277541895291No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT16960.11244566673119917No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16930.11224676519806616No Hit
CAACTATGGTGATGGTCGGGTCTTCGGAGCTGGGACCAGACTCACCGTCC16490.10932954271211523No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC16450.10906434066793787No Hit
GTCCAGGAATATTTGTTGGTTGTACTATAAATCAGTGCCCTGGGTACTTG16340.10833503504645015No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA16110.10681012329243035No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16040.10634601971511996No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG16010.10614711818198695No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15910.10548411307154355No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15810.10482110796110017No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15750.10442330489483413No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA15740.1043570043837898No Hit
TCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCAC15710.10415810285065677No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG15560.1031635951849917No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG15520.10289839314081434No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCC15230.1009756783205285No Hit
GCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGACTATTACTG15160.10051157474321812No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15090.10004747116590774No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)