FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001318887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001318887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507549
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57960.3844651152300854No Hit
GATTTATAGTACAACCAACAAATATTCCTGGACCCCTGCCCGGTTCTCAG51090.33889445716192307No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT49090.3256278900387317No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA44030.2920634752170576No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT43580.28907849761433957No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC41920.2780672469020907No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA41090.2725616215459663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40610.2693776454364004No Hit
CTATAAATCAGTGCCCTGGGTACTTGTCCAGGTTTTTGCTGGAACCAGTT39120.25949405292962285No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC38880.25790206487483985No Hit
ACCTAGGACGGTGAGTCTGGTCCCAGCTCCGAAGACCCGACCATCACCAT36420.24158418731331455No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC36220.24025753060099542No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC35930.23833387836813263No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC35730.2370072216558135No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA34790.23077193510791355No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT34050.22586330527233278No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC32730.21710737097102648No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32480.21544905008062756No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG32370.21471938888885206No Hit
GACCTAGGACGGTGAGTCTGGTCCCAGCTCCGAAGACCCGACCATCACCA30220.20045782923142136No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAG29560.1960798620807682No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT28930.1919008934369629No Hit
GACTATTACTGCCTACTCAACTATGGTGATGGTCGGGTCTTCGGAGCTGG28730.19057423672464377No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA28100.1863952680808385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27780.18427261734112788No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT27110.17982831735485877No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG26950.17876699198500345No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25310.16788840694398655No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG24120.1599947995056877No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23600.15654549205365798No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT22960.15230019057423674No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCA22730.1507745353550697No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG22200.147258895067424No Hit
GCCTTGGGCTGACCTAGGACGGTGAGTCTGGTCCCAGCTCCGAAGACCCG22120.14672823238249635No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG21740.14420758462909No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG21560.14301359358800278No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA21420.14208493388937937No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA21370.1417532697112996No Hit
GCCTGAGGACGAGGCTGACTATTACTGCCTACTCAACTATGGTGATGGTC21100.13996228314966877No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT20670.13710997121818264No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAG20530.13618131151955923No Hit
TTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG20380.13518631898531988No Hit
CCATAGTTGAGTAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACC19970.13246667272506565No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG19720.13080835183466671No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC19220.1274917100538689No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG18130.12026143097172962No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18130.12026143097172962No Hit
ATCCAAACTGGTTCCAGCAAAAACCTGGACAAGTACCCAGGGCACTGATT17930.11893477425941049No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT17820.11820511306763494No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA17730.11760811754709134No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG17620.11687845635531581No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17490.11601612949230838No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC17030.11296481905397437No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG16900.11210249219096692No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16860.11183716084850309No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA16700.11077583547864779No Hit
CAACTATGGTGATGGTCGGGTCTTCGGAGCTGGGACCAGACTCACCGTCC16630.1103115056293361No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCC16430.10898484891701697No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG16310.10818885488962549No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG16240.10772452504031378No Hit
GCACTGGAGCAGTCACCAGTGATTACTATCCAAACTGGTTCCAGCAAAAA15950.10580087280745104No Hit
GTCCAGGAATATTTGTTGGTTGTACTATAAATCAGTGCCCTGGGTACTTG15870.10527021012252338No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG15870.10527021012252338No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGAGTCTGGTC15830.10500487878005957No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15650.10381088773897235No Hit
TCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCAC15580.10334655788966063No Hit
ACTATGGTGATGGTCGGGTCTTCGGAGCTGGGACCAGACTCACCGTCCTA15490.10274956236911703No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG15430.1023515653554213No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC15260.10122390714995001No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC15110.10022891461571068No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)