FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001318888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001318888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507549
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTTATAGTACAACCAACAAATATTCCTGGACCCCTGCCCGGTTCTCAG54260.35992196605218135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53490.35481433770975274No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT47440.3146829721620989No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT42930.28476686329930234No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40880.2711686319980312No Hit
CTATAAATCAGTGCCCTGGGTACTTGTCCAGGTTTTTGCTGGAACCAGTT39870.26446901560081965No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC38750.2570397380118325No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA37620.24954412758722935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37410.24815113803929426No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA36450.2417831858201624No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC33860.22460298139562962No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT33790.22413865154631787No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC33330.2210873411079839No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC33240.22049034558744027No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC32650.21657670828609885No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC31710.21034142173819886No Hit
ACCTAGGACGGTGAGTCTGGTCCCAGCTCCGAAGACCCGACCATCACCAT31440.20855043517656804No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29730.19720752028623947No Hit
GACTATTACTGCCTACTCAACTATGGTGATGGTCGGGTCTTCGGAGCTGG29200.19369187999859375No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG28860.1914365635876512No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA26880.1783026621356918No Hit
GACCTAGGACGGTGAGTCTGGTCCCAGCTCCGAAGACCCGACCATCACCA26200.17379202931380672No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT25790.1710723830535525No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG25670.170276389026161No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAG25420.1686180681357621No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA25300.1678220741083706No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT25040.16609742038235573No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23990.15913247264268027No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA23990.15913247264268027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23910.1586018099577526No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG23580.15641282638242604No Hit
TTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG22980.15243285624546865No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22900.151902193560541No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCA22000.14593223835510485No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG21830.1448045801496336No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT21330.14148793836883578No Hit
GCCTTGGGCTGACCTAGGACGGTGAGTCTGGTCCCAGCTCCGAAGACCCG21250.14095727568390812No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG20990.13923262195789324No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG20900.1386356264373496No Hit
GCCTGAGGACGAGGCTGACTATTACTGCCTACTCAACTATGGTGATGGTC20290.13458932346477628No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAG19570.1298133593004274No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA19540.12961436079357952No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA19150.1270273802045572No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18720.12417506827307105No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT18480.12258308021828809No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG18390.1219860846977445No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG18220.12085842649227321No Hit
CCATAGTTGAGTAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACC18070.11986343395803388No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC17340.11502113695806902No Hit
ATCCAAACTGGTTCCAGCAAAAACCTGGACAAGTACCCAGGGCACTGATT17220.11422514293067754No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC17080.11329648323205414No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC16770.1112401653279595No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT16640.11037783846495205No Hit
TCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCAC16550.10978084294440844No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA16380.10865318473893718No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16360.10852051906770525No Hit
CAACTATGGTGATGGTCGGGTCTTCGGAGCTGGGACCAGACTCACCGTCC16020.10626520265676273No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15940.10573453997183507No Hit
GCACTGGAGCAGTCACCAGTGATTACTATCCAAACTGGTTCCAGCAAAAA15920.10560187430060317No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15890.1054028757937553No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG15730.10434155042389999No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15650.10381088773897235No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG15500.10281589520473298No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG15470.10261689669788512No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA15360.10188723550610959No Hit
GTCCAGGAATATTTGTTGGTTGTACTATAAATCAGTGCCCTGGGTACTTG15360.10188723550610959No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)