FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001318913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001318913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644163
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGA105140.6394743100288718No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT78690.47860218238702606No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT61200.3722258681164824No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC59450.36158215456739995No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC59180.3599399816198272No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC54680.3325704324936153No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC52500.31931140647247264No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT51270.31183039637797466No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGCGTCACCCTCTCCT48110.2926108907693459No Hit
CTGTTATCCCAGCCAGGTTCAGTGGCAGTGGGTCCGAGACAGAGTTCACT45450.27643244617474055No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44680.2717492122131443No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41450.25210395806255215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38460.2339184131986914No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38300.23294527367420387No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG37590.22862696703429042No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT37330.22704561530699813No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGGAGTTTCTTAGCCTGGTACCAGC34330.20879924922285686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34190.20794775213893024No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33000.20071002692555423No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32060.19499283221918995No Hit
TTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCAT30360.1846532247715099No Hit
GTCTGTGTCTCCAGGGGAAAGCGTCACCCTCTCCTGCAGGGCCAGTCAGA30040.1827069457225348No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29800.1812472364358035No Hit
GGATAACAGTGGCCCTGGTGGAAACACCATAGATGAGGAGCCTGGGAGCC29130.17717221467701197No Hit
GGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGC28150.17121173508952578No Hit
GTCCGAGACAGAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATT26900.16360908255446693No Hit
GTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCTCCGCCGAAAGTG26390.1605072003201629No Hit
TTATTACTGTCAGCAGTATAATAACTGGCCCCTCACTTTCGGCGGAGGGA25900.15752696052641982No Hit
TCTATGGTGTTTCCACCAGGGCCACTGTTATCCCAGCCAGGTTCAGTGGC25180.1531478326662259No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA25130.15284372656482356No Hit
CATCTATGGTGTTTCCACCAGGGCCACTGTTATCCCAGCCAGGTTCAGTG24740.1504716989738852No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24550.14931609578855623No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG24500.1490119896871539No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC23900.14536271647032564No Hit
CACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGC23700.1441462920647162No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG23670.1439638284038748No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23460.1426865827779849No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA23110.14055784006816843No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA23060.14025373396676608No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22830.13885484590031524No Hit
GTACATGGGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAG22580.13733431539330346No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCC22330.1358137848862917No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21940.13344175729535332No Hit
GTCTCGGACCCACTGCCACTGAACCTGGCTGGGATAACAGTGGCCCTGGT21870.13301600875339No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCCCTCACTTTCGGCGGAGG20280.12334543472879514No Hit
GGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCG20240.12310214984767325No Hit
GAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTA19670.1196353402916864No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19460.11835809466579651No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGA19400.1179931673441137No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC18910.11501292755037061No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18810.11440471534756591No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18760.11410060924616355No Hit
CTCCCAGGCTCCTCATCTATGGTGTTTCCACCAGGGCCACTGTTATCCCA18540.11276254239999318No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC18190.11063379969017671No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18160.1104513360293353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18040.10972148138596964No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT17820.10838341453979927No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG17330.10540317474605619No Hit
GGAGAAACAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG17150.10430839278100773No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16650.10126733176698417No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA16590.10090240444530134No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)