FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001318914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001318914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644163
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGA104650.6364940702351287No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT78280.47610851235552676No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT60430.3675426341548861No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC53020.3224741099270571No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52320.31821662450742416No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50730.30854605048282924No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC48290.29370567273439435No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT47260.2874410870455058No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGCGTCACCCTCTCCT46940.28549480799653076No Hit
CTGTTATCCCAGCCAGGTTCAGTGGCAGTGGGTCCGAGACAGAGTTCACT42670.2595241469367697No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39400.23963560790505567No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT36810.22388291185241366No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36300.22078102961810964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35700.21713175640128138No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG35600.21652354419847666No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34610.21050224339071005No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32650.19858128421573773No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGGAGTTTCTTAGCCTGGTACCAGC32390.1969999324884455No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG31510.19164766510376402No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30470.18532225819459508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30210.18374090646730282No Hit
GGATAACAGTGGCCCTGGTGGAAACACCATAGATGAGGAGCCTGGGAGCC28030.17048188044616014No Hit
TTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCAT27820.16920463482027026No Hit
GTCCGAGACAGAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATT27120.1649471494006373No Hit
GTCTGTGTCTCCAGGGGAAAGCGTCACCCTCTCCTGCAGGGCCAGTCAGA26920.1637307249950279No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26240.15959488201595584No Hit
GTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCTCCGCCGAAAGTG26210.15941241835511444No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26020.15825681516978546No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC25790.15685792710333465No Hit
TTATTACTGTCAGCAGTATAATAACTGGCCCCTCACTTTCGGCGGAGGGA25570.15551986025716427No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA24610.1496810231102391No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA23720.14426793450527717No Hit
GTCTCGGACCCACTGCCACTGAACCTGGCTGGGATAACAGTGGCCCTGGT23600.1435380798619115No Hit
CACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGC23460.1426865827779849No Hit
GGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGC23310.14177426447377783No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG23180.14098358861013172No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC22520.13696938807162062No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA22460.13660446074993782No Hit
TCTATGGTGTTTCCACCAGGGCCACTGTTATCCCAGCCAGGTTCAGTGGC22240.13526639390376743No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG22120.1345365392604018No Hit
CATCTATGGTGTTTCCACCAGGGCCACTGTTATCCCAGCCAGGTTCAGTG22060.134171611938719No Hit
GTACATGGGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAG21930.13338093607507287No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCCCTCACTTTCGGCGGAGG21760.13234697533030484No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCC20920.12723799282674528No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20740.1261432108616968No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19640.11945287663084499No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGA19540.11884466442804029No Hit
GAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTA18770.11416143046644402No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18670.1135532182636393No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18640.1133707546027979No Hit
CTCCCAGGCTCCTCATCTATGGTGTTTCCACCAGGGCCACTGTTATCCCA18370.11172858165522517No Hit
GGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCG17990.10941737528456728No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC17980.1093565540642868No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17410.10588974450829997No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT17350.10552481718661715No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17320.10534235352577573No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC17210.10467332010269054No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)