FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001318915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001318915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650785
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGA104790.6347889034610806No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT79670.4826188752623752No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT63360.38381739596616155No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58750.3558912880841539No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC57410.34777393785380895No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC55290.3349315628625169No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC52850.3201507161744261No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT51290.31070066665253193No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGCGTCACCCTCTCCT47570.288165933177246No Hit
CTGTTATCCCAGCCAGGTTCAGTGGCAGTGGGTCCGAGACAGAGTTCACT44620.2702956472223821No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44400.2689629479308329No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41900.2538186377995923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38770.2348579615152791No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG37570.22758869265228363No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG36990.2240752127018358No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT36710.22237904996713684No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGGAGTTTCTTAGCCTGGTACCAGC34960.21177803287526845No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33690.20408472332859823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33090.20045008889710048No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31720.1921510069451806No Hit
TTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCAT30370.18397307947431069No Hit
GGATAACAGTGGCCCTGGTGGAAACACCATAGATGAGGAGCCTGGGAGCC29740.1801567133212381No Hit
GTCTGTGTCTCCAGGGGAAAGCGTCACCCTCTCCTGCAGGGCCAGTCAGA29060.17603746096554063No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28860.1748259161550414No Hit
GTCCGAGACAGAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATT28410.1720999403314181No Hit
GGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGC27090.16410374458212307No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA25850.15659216675702772No Hit
GTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCTCCGCCGAAAGTG25760.15604697159230305No Hit
TTATTACTGTCAGCAGTATAATAACTGGCCCCTCACTTTCGGCGGAGGGA25510.15453254057917898No Hit
TCTATGGTGTTTCCACCAGGGCCACTGTTATCCCAGCCAGGTTCAGTGGC25000.15144310131240593No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24990.15138252407188096No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG24970.15126136959083103No Hit
CACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGC24730.14980751581823193No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC24570.14883827996983254No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG23880.14465845037361014No Hit
GTACATGGGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAG23740.14381036900626065No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA23690.14350748280363584No Hit
CATCTATGGTGTTTCCACCAGGGCCACTGTTATCCCAGCCAGGTTCAGTG23570.1427805559173363No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA22880.1386007263211139No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22810.13817668563743915No Hit
GTCTCGGACCCACTGCCACTGAACCTGGCTGGGATAACAGTGGCCCTGGT22680.13738918151061466No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22030.13345166087649207No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21490.13018048988814412No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCC21120.12793913198872053No Hit
GGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCG20810.12606123753244666No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCCCTCACTTTCGGCGGAGG20430.1237593023924981No Hit
GAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTA20070.12157852173359947No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19580.11861023694787631No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19300.11691407421317737No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC19050.1153996432000533No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGA19010.11515733423795346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18720.11340059426272954No Hit
CTCCCAGGCTCCTCATCTATGGTGTTTCCACCAGGGCCACTGTTATCCCA18350.11115923636330595No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT18340.11109865912278098No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18170.11006884603385661No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18150.1099476915528067No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC17580.10649478884288384No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC16740.101406300638787No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16600.10055821927143754No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG16600.10055821927143754No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA16530.1001341785877628No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC16520.10007360134723783No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)