FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001318916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001318916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650785
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATGA104230.6313965779916828No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT76250.4619014590028381No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT59700.36164612593402534No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC53830.3260872857458724No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52120.31572857761610384No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50150.3037948612326863No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC46750.28319859945419906No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT46500.28168416844107497No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGCGTCACCCTCTCCT45540.2758687533506786No Hit
CTGTTATCCCAGCCAGGTTCAGTGGCAGTGGGTCCGAGACAGAGTTCACT42650.2583619308389645No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37590.22770984713333353No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT36860.2232877085750113No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG36650.22201558652398706No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36520.22122808239716257No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34940.2116568783942185No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33520.20305491023967387No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32450.19657314550350288No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGGAGTTTCTTAGCCTGGTACCAGC32110.19451351932565417No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31550.19112119385625626No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG30690.18591155117110952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29980.1816105670938372No Hit
TTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCAT28960.175431688560291No Hit
GGATAACAGTGGCCCTGGTGGAAACACCATAGATGAGGAGCCTGGGAGCC28120.17034320035619416No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26830.16252873632847403No Hit
GTCCGAGACAGAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATT26520.1606508418722002No Hit
GTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCCCTCCGCCGAAAGTG26430.16010564670747554No Hit
GTCTGTGTCTCCAGGGGAAAGCGTCACCCTCTCCTGCAGGGCCAGTCAGA26180.15859121569435147No Hit
TTATTACTGTCAGCAGTATAATAACTGGCCCCTCACTTTCGGCGGAGGGA25850.15659216675702772No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25700.15568350814915327No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC25280.15313926404710487No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA24630.1492017434129823No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA24150.1462940358677841No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC23460.1421142062715617No Hit
CACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGC23440.1419930517905118No Hit
GTCTCGGACCCACTGCCACTGAACCTGGCTGGGATAACAGTGGCCCTGGT23290.14108439318263735No Hit
CATCTATGGTGTTTCCACCAGGGCCACTGTTATCCCAGCCAGGTTCAGTG23100.13993342561266306No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG23020.13944880768846335No Hit
GTACATGGGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAG22640.1371468725485148No Hit
TCTATGGTGTTTCCACCAGGGCCACTGTTATCCCAGCCAGGTTCAGTGGC22540.13654110014326518No Hit
GGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGC22530.1364805229027402No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA22320.135208400851716No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG21710.1315131891796933No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCACCTTGGTCC21130.1279997092292455No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCCCTCACTTTCGGCGGAGG20440.12381987963302307No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19800.1199429362394255No Hit
GAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTA19660.11909485487207601No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19280.11679291973212744No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGA19230.11649003352950263No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18840.11412752114902909No Hit
CTCCCAGGCTCCTCATCTATGGTGTTTCCACCAGGGCCACTGTTATCCCA18480.11194674049013047No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18320.11097750464173105No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC18190.11019000051490654No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC17200.10419285370293528No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17160.10395054474083541No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGT16750.10146687787931197No Hit
GGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCG16530.1001341785877628No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)