FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001319042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2777773
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT91870.33073256886001845No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA67720.24379241932296122No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52240.18806432347063637No Hit
TTATTACACCTCCTGATTTTCACTCCTTGAGTTTTATTTTCTCCCCTTGT44130.15886827325343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40140.1445042485473075No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC37610.13539623288152056No Hit
GTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACCTTATG33220.1195922056985938No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCC33130.11926820514131284No Hit
TTCATACTCTAAGTATCAAAATCTTCCAATTATCATGCTCACCTGAAAGA32030.11530819833010111No Hit
TAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAATCAGGT31690.11408419622481751No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCTCGGCACTCAGG30380.10936818811328355No Hit
CTCTTAGGAATACAGTTTCTAGCATTAAACAAATAAACAAGGGGAGAAAA29310.10551618148783215No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT28850.10386017863950726No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC28770.10357217814414642No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)