FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001319073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447531
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT240670.9833174738134063Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT109110.44579619216263244Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT93900.38365193331565567Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC80080.32718686709177536Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT69700.28477678117253674Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT63970.26136543316509575Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63920.26116114566066784No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC61570.251559632952555Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC55260.22577854989375007Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51090.2087409720244606No Hit
GAGTAGGAGTGTCCACTGGTACCAGCAGAAGCCAGGCCTGGCCCCTAAGT49360.20167262437125413No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC49250.2012231918615127No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG46200.18876165409140885Clontech Universal Primer Mix Long (96% over 25bp)
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC46010.1879853615745827No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG43660.17838384886646994No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC43510.17777098635318614Clontech Universal Primer Mix Long (96% over 25bp)
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTG43380.17723983884167352No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA41450.16935434117075535Clontech Universal Primer Mix Long (96% over 25bp)
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA40770.16657603111053546No Hit
CTCCTACTCCCAATGTTTTTTGCCGCACAGGTAATTCTGGCCGTCTTTCC38950.15913996594935875No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA38490.15726052090862178Clontech Universal Primer Mix Long (96% over 25bp)
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA38330.1566068008944524No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC35560.145289273149145No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA34470.1408358055526161No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG34380.1404680880446458No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33930.13862950050479445No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33900.13850692800213765No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32420.13246001787107087No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC31740.129681707810851Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT31510.12874198529048253No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31500.12870112778959694No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29480.12044791261070852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29260.1195490475912256No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA28640.11701588253631927No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28340.11579015750975168No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG28320.1157084425079805No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT27920.11407414247255702Clontech Universal Primer Mix Long (96% over 25bp)
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT27840.11374728246547235No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC27620.11284841744598946Clontech Universal Primer Mix Long (96% over 25bp)
CTGTAATTATCCCACACCTGACAGTAAAAGTCGGCCTCATCCCCGGCCGC27380.11186783742473537No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA27000.1103152523910831No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT26960.11015182238754075No Hit
GCCCTCAGGGGTCCCTGAGCGATTCTCAGGCTCCAACTCTGGGAACACGG26770.10937552987071461No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA26720.10917124236628668No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA26660.10892609736097315No Hit
CCCAATGATCACTGTAATTATCCCACACCTGACAGTAAAAGTCGGCCTCA26520.10835409234857495No Hit
GAATTACCTGTGCGGCAAAAAACATTGGGAGTAGGAGTGTCCACTGGTAC26430.10798637484060467No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA25850.10561663978924066No Hit
TAATTACAGTGATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC25620.10467691726887217No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC25470.10406405475558839No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT25010.10218460971485141No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)