FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001319074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447531
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT223790.9143500123185365Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT117450.47987134790121144Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT101200.4134779089621337Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC88060.35979115279847323Clontech Universal Primer Mix Long (96% over 25bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT65860.26908750083247157Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT65200.26639090577402286Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57610.23538006260186284No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC54470.22255080732378874Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC51680.21115156457671017Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAGGAGTGTCCACTGGTACCAGCAGAAGCCAGGCCTGGCCCCTAAGT47980.1960342892490432No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC47630.1946042767180477No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC45220.1847576190046214Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43560.17797527385761408No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTG41710.17041663619378059No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC41400.16915005366632743No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG41220.16841461865038687No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG40880.16702546362027693Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA39200.16016140347149843Clontech Universal Primer Mix Long (96% over 25bp)
CTCCTACTCCCAATGTTTTTTGCCGCACAGGTAATTCTGGCCGTCTTTCC37950.15505421586080012No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA37450.15301134081652082No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA36740.1501104582536442No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36070.1473730056943099No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35480.1449624131420603No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA34010.13895636051187912Clontech Universal Primer Mix Long (96% over 25bp)
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC32940.13458460791712137No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA31830.1300494253188213No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA31510.12874198529048253No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31190.12743454526214376No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG30880.12616796273469058No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC30620.12510566771166534Clontech Universal Primer Mix Long (96% over 25bp)
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT28780.11758788754871748No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC28170.11509557999469669Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28090.11476871998761201No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT28090.11476871998761201Clontech Universal Primer Mix Long (96% over 25bp)
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27970.11427842997698498No Hit
GCCCTCAGGGGTCCCTGAGCGATTCTCAGGCTCCAACTCTGGGAACACGG26990.1102743948901975No Hit
CTGTAATTATCCCACACCTGACAGTAAAAGTCGGCCTCATCCCCGGCCGC26400.10786380233794791No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC26020.10631121730429564No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT25930.10594349979632536No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA25870.10569835479101183No Hit
CCCAATGATCACTGTAATTATCCCACACCTGACAGTAAAAGTCGGCCTCA25840.10557578228835508No Hit
GAATTACCTGTGCGGCAAAAAACATTGGGAGTAGGAGTGTCCACTGGTAC25770.10528977978215598No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG25650.10479948977152895No Hit
TAATTACAGTGATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC24910.10177603470599556No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA24790.10128574469536852No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA24590.10046859467765679No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA24510.10014173467057211Clontech Universal Primer Mix Long (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)