FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001319075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452926
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT239420.9760587967186942Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT111670.4552522171480102Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT94490.38521341450985475Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC79140.32263508968472754Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT68960.28113363387236306Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64990.26494888145830736No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT63360.2583037564117303Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC60430.2463588383832207Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC56300.22952180375600406Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51700.21076869012762717No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC50720.20677346157201645No Hit
GAGTAGGAGTGTCCACTGGTACCAGCAGAAGCCAGGCCTGGCCCCTAAGT48060.1959292697782159No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG45500.18549275436764093Clontech Universal Primer Mix Long (96% over 25bp)
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC45440.18524814853770558No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG43600.17774690308635482No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTG43260.17636080338338786No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC42710.17411858327564714Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA42330.1725694130193899Clontech Universal Primer Mix Long (96% over 25bp)
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA39720.16192905941720215No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA38410.156588498796947No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA38360.1563846606053342Clontech Universal Primer Mix Long (96% over 25bp)
CTCCTACTCCCAATGTTTTTTGCCGCACAGGTAATTCTGGCCGTCTTTCC37750.15389783466765813No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC35890.14631505393966227No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG35090.1430536428738576No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34750.14166754317089061No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA34500.14064835221282665No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34190.13938455542482733No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32660.1331471067614759No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32320.1317610070585089No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC31790.1296003222274133Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT31140.12695042573644702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30250.1233221059257393No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29780.12140602692457905No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG28690.1169623543474202No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT28260.11520934589955016No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT27980.11406785202651853Clontech Universal Primer Mix Long (96% over 25bp)
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA27600.11251868177026131No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27360.11154025845051992No Hit
GCCCTCAGGGGTCCCTGAGCGATTCTCAGGCTCCAACTCTGGGAACACGG27310.11133642025890711No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA27260.11113258206729433No Hit
CTGTAATTATCCCACACCTGACAGTAAAAGTCGGCCTCATCCCCGGCCGC27160.11072490568406873No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC27070.11035799693916572Clontech Universal Primer Mix Long (96% over 25bp)
CCCAATGATCACTGTAATTATCCCACACCTGACAGTAAAAGTCGGCCTCA26770.10913496778948897No Hit
GAATTACCTGTGCGGCAAAAAACATTGGGAGTAGGAGTGTCCACTGGTAC26600.10844191793800548No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT26450.1078304033631671No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA26270.10709658587336104No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA25810.10522127451052335No Hit
TAATTACAGTGATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC25730.1048951334039429No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTGT25360.10338673078600823No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24710.10073683429504192No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)