FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001319197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305618
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT120590.5230267980211813Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTTTATACAGCTCCGACAATAGGAACTACTTCGCTTGGTACCAGCAGAG63830.27684551387090145No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61960.26873489016827595No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT57880.2510389839080021Clontech Universal Primer Mix Long (96% over 25bp)
ATATTATAGTATTCCAATCACTTTCGGCGGAGGGACCAAGGTGGAGATCA56520.24514034848791083No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT55530.24084648888063853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55360.2401091594531271No Hit
CTCCTAGGTTGCTCATTTACTGGGCATCTACTCGGGAATCCGGGGTCCCT48720.21130993946091675No Hit
ACCTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCGAAGTAGTTCCTA48150.20883771726279027No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46580.2020282631381261Clontech Universal Primer Mix Long (96% over 25bp)
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44420.19265984217680465No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42310.18350828281181011No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC39870.17292543691105813Clontech Universal Primer Mix Long (96% over 25bp)
GTATAAAAGAGTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCA34440.14937426754995842No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT33880.14694541767109728Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33580.145644248093136No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCT32300.14009259122716772No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31510.13666617800520295No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG30740.1333265094217689No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30520.13237231839793062Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30240.13115789345850007Clontech Universal Primer Mix Long (96% over 25bp)
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28840.12508576876134728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28700.12447855629163201No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28240.12248342960542466Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27760.12040155828068656No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27300.11840643159447924No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT25550.11081627572303826No Hit
GTACCAGCAGAGACCAGGACAGCCTCCTAGGTTGCTCATTTACTGGGCAT25530.1107295310845075No Hit
GACCAGGACAGCCTCCTAGGTTGCTCATTTACTGGGCATCTACTCGGGAA25220.1093849891872808No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23680.10270565202041275Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGC23590.10231530114702435No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)