FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001319198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305618
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT112490.4878952194162259Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT60660.26309648866377694Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57300.24852338939061022No Hit
ATATTATAGTATTCCAATCACTTTCGGCGGAGGGACCAAGGTGGAGATCA54730.23737670333940836No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT53570.2323455143046246No Hit
CTTTTATACAGCTCCGACAATAGGAACTACTTCGCTTGGTACCAGCAGAG53510.23208528038903237No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48810.21170029033430515Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47710.2069293352151137No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCT46640.20228849705371835No Hit
ACCTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCGAAGTAGTTCCTA43530.1887997057621861No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41190.17865058305408787Clontech Universal Primer Mix Long (96% over 25bp)
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40240.1745302127238771No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39760.17244834139913898No Hit
CTCCTAGGTTGCTCATTTACTGGGCATCTACTCGGGAATCCGGGGTCCCT37840.1641208561001866No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT33700.1461647159243205Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATAAAAGAGTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCA33280.14434307851517467No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT31960.13861793237214493Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31520.13670955032446833No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27850.12079190915407496No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27540.11944736725684828Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26850.11645467722753726No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26330.11419931662573765No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25920.1124210515358572No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT25910.11237767921659184No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG25740.11164034978908041No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25170.10916812759095393Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24730.10725974554327733No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24290.10535136349560076No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)