FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001319199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2302773
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT122250.5308816804782756Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64920.28192097093373947No Hit
CTTTTATACAGCTCCGACAATAGGAACTACTTCGCTTGGTACCAGCAGAG64500.2800970829517282No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58230.2528690409345602Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55810.24235997208582868No Hit
ATATTATAGTATTCCAATCACTTTCGGCGGAGGGACCAAGGTGGAGATCA55500.24101376905148708No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT55020.23892932564347416No Hit
CTCCTAGGTTGCTCATTTACTGGGCATCTACTCGGGAATCCGGGGTCCCT49730.21595702225099914No Hit
ACCTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCGAAGTAGTTCCTA49050.21300406075631423No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46360.2013224924905755Clontech Universal Primer Mix Long (96% over 25bp)
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44260.19220305258051923No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41870.18182426144478853No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38630.1677542684407017Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35500.15416196038428454Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATAAAAGAGTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCA34140.14825603739491475No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33760.1466058530302379No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCT32480.14104733727553692No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32010.13900631977185768No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30260.13140678651347745Clontech Universal Primer Mix Long (96% over 25bp)
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG30040.13045141661813822No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29600.12854067682745976Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28940.1256745671414421No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28520.12385067915943082No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28240.12263475383809No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC27940.12133197670808195Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27240.11829216340472985No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT25880.1123862404153601No Hit
GACCAGGACAGCCTCCTAGGTTGCTCATTTACTGGGCATCTACTCGGGAA25500.11073605605068325No Hit
GTACCAGCAGAGACCAGGACAGCCTCCTAGGTTGCTCATTTACTGGGCAT25490.11069263014634964No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23530.10218115289696379Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGC23400.10161661614062699No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)