FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001319200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2302773
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT110690.4806813350686325Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT61960.26906690325099347Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57920.25152283790021857No Hit
ATATTATAGTATTCCAATCACTTTCGGCGGAGGGACCAAGGTGGAGATCA55220.23979784373014623No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT54660.23736599308746453No Hit
CTTTTATACAGCTCCGACAATAGGAACTACTTCGCTTGGTACCAGCAGAG51100.2219063711447025No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT50380.2187797060326832Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49800.21626100358133432No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCT47990.2084009148969525No Hit
ACCTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCGAAGTAGTTCCTA43610.18938036879883516No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC42140.18299676086179575Clontech Universal Primer Mix Long (96% over 25bp)
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39860.17309565467373467No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39220.1703163967963842No Hit
CTCCTAGGTTGCTCATTTACTGGGCATCTACTCGGGAATCCGGGGTCCCT36500.15850455081764464No Hit
GTATAAAAGAGTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCA34900.15155640612426843No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32650.14178557764920816Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32350.1404828005192001Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31120.13514141428616716No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26840.11655512723138582No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC26730.1160774422837162Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26720.11603401637938258No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26290.11416670249303774No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25130.10912929759034No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG25120.1090858716860064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24800.10769624274733117No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24620.10691457646932633Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24410.1060026324783207No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT24360.10578550295665269No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA23460.1018771715666286No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)