FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001319201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2239888
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT139220.6215489345895866No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC107030.477836391819591No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC100630.4492635346053017No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT93580.41778874658018617No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG80820.36082161250919687No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC80050.3573839406256027No Hit
GACTTATAGTACCCTCAACTTCGGCGAAGGGACCTTTACTTTCGGCGGAG78640.3510889830205796No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG74330.33184694949033167No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG69850.3118459494403291No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGTCAG67790.3026490610244798No Hit
AGTCAGAACATTGTCAGATATTTAAATTGGTATCAGCACAAACCGGGGAA67530.3014882887001493No Hit
CACTATCAGTAGTCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAAG61030.27246898059188673No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA60030.268004471652154No Hit
CTATAAGTCTCTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGGAGACT60010.26791518147335935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59120.26394176851699724No Hit
GATCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTTCAGTG58400.26072732208038973No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT56920.25411984884958533No Hit
CTCCTGATCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTT55070.24586050731107983No Hit
ACTTACTACTGTCAAGAGACTTATAGTACCCTCAACTTCGGCGAAGGGAC54840.24483367025494135No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54400.24286928632145893No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG52190.23300272156464966No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT52070.23246698049188175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50330.22469873493674683No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG50140.22385047823819762No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT50140.22385047823819762No Hit
GGGTGGACATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG48990.218716292957505No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA48840.2180466166165451No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGGAGACTACTGATAGTGAGACTGA48120.21483217017993755No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT47600.21251062553127656No Hit
GATCAGGAGTCTAGGGGCTTTCCCCGGTTTGTGCTGATACCAATTTAAAT46810.20898366346888772No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC45410.20273335095326192No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG44310.197822391119556No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATGTCCACCCTGGTCC43400.19375968798439921No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT43140.19259891566006873No Hit
CCCCTAGACTCCTGATCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCA43040.19215246476609546No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCAGTCTCACTATC42450.18951840449165314No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA42370.18916124377647453No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT41640.18590215225046966No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG40820.18224125491988885No Hit
GTGCAGCCACAGTTCCTTTGATGTCCACCCTGGTCCCTCCGCCGAAAGTA40440.18054474152279043No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA39500.17634810311944168No Hit
GCATAGATCAGGAGTCTAGGGGCTTTCCCCGGTTTGTGCTGATACCAATT39480.17625881294064705No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA39140.17474087990113793No Hit
GTCAAGAGACTTATAGTACCCTCAACTTCGGCGAAGGGACCTTTACTTTC38690.1727318508782582No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA38480.17179430400091433No Hit
GTCTAGGGGCTTTCCCCGGTTTGTGCTGATACCAATTTAAATATCTGACA38450.17166036873272233No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37980.16956204953104798Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC37910.16924953390526667No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA37540.1675976655975656No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA36750.16407070353517678No Hit
ATCTGGGACAGATTTCAGTCTCACTATCAGTAGTCTCCAACCTGAAGATT36630.16353496246240884No Hit
GTTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAG35860.16009729057881464No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA35220.15724000485738573No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34090.15219510975548778No Hit
ATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTTCAGTGGCAGT33490.14951640439164815No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA33160.14804311644153637No Hit
TGTCCACCCTGGTCCCTCCGCCGAAAGTAAAGGTCCCTTCGCCGAAGTTG32650.14576621688227268No Hit
TCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTTCAGTGGC32520.14518583072010743No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC32510.1451411856307101No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT31520.14072132178037472No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA31370.14005164543941484No Hit
GCACAAACCGGGGAAAGCCCCTAGACTCCTGATCTATGCTGCGTCCAGTC31090.13880158293628966No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC31050.13862300257870036No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG30950.1381765516847271No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG30610.13665861864521797No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT30590.13656932846642333No Hit
CTGATAGTGAGACTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGA30450.13594429721486076No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA30340.13545320123149013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29860.13331023694041844No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29600.13214946461608795No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC29320.1308994021129628No Hit
ATAGTACCCTCAACTTCGGCGAAGGGACCTTTACTTTCGGCGGAGGGACC29250.1305868864871815No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA29210.13040830612959217No Hit
GATTTCAGTCTCACTATCAGTAGTCTCCAACCTGAAGATTTTGCAACTTA28990.129426114162851No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA28820.12866714764309645No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC28630.12781889094454724No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC28400.12679205388840872No Hit
ACCTTGATGAGACCCCACTTTGCAGACTGGACGCAGCATAGATCAGGAGT28350.1265688284414221No Hit
GACCAGGGTGGACATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCT28260.12616702263684612No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG28090.12540805611709155No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC27990.12496160522311829No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC27610.12326509182601986No Hit
CTGCTACTCTGGCTCCGAGGTGTCAGATGTGACATCCAGATGACCCAGTC27520.12286328602144393No Hit
CCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG27480.12268470566385462No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27110.12103283735615353No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT26750.11942561413784976No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA26560.11857735743930053No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC26200.11697013422099677No Hit
CTATCAGTAGTCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAAGAG25900.11563078153907695No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG25780.11509504046630903No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA25660.11455929939354112No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25500.11384497796318388No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC25470.1137110426949919No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC25410.11344317215860793No Hit
TCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAAGAGACTTATAGTA25390.11335388197981328No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCAGTCTCACTATCAGTAGTC25090.11201452929789346No Hit
TCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGAT24950.11138949804633089No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT24920.1112555627781389No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC24370.10880008286128592No Hit
CTACAAGACCACACCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT24360.1087554377718886No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC24350.10871079268249127No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24300.10848756723550464No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG24260.10830898687791532No Hit
ACCCTCAACTTCGGCGAAGGGACCTTTACTTTCGGCGGAGGGACCAGGGT24020.10723750473237949No Hit
CCCTGGTCCCTCCGCCGAAAGTAAAGGTCCCTTCGCCGAAGTTGAGGGTA23600.10536241097769174No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT23450.10469273463673183No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG23400.10446950918974521No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGTC23360.10429092883215589No Hit
TCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTG23160.10339802704420935No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGAGACCCCACTTTGCAGACT22570.10076396676976707No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)