FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001319202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2239888
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT148010.6607919681698371No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC118620.529580050431093No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC116030.5180169722771852No Hit
GACTTATAGTACCCTCAACTTCGGCGAAGGGACCTTTACTTTCGGCGGAG89890.40131470859257246No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT86840.38769795632638776No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG76390.34104383790618104No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC76060.3395705499560692No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG75590.33747223075439486No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT70630.3153282664133207No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA68960.30787253648396706No Hit
CTATAAGTCTCTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGGAGACT67050.2993453244090776No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC66860.29849706771052836No Hit
CTCCTGATCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTT66050.29488081546934486No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG63270.282469480616888No Hit
CACTATCAGTAGTCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAAG62960.28108548284557083No Hit
AGTCAGAACATTGTCAGATATTTAAATTGGTATCAGCACAAACCGGGGAA62860.2806390319515976No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGTCAG62330.27827284221353926No Hit
GATCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTTCAGTG58090.2593433243090726No Hit
ACTTACTACTGTCAAGAGACTTATAGTACCCTCAACTTCGGCGAAGGGAC57590.2571110698392063No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT56080.2503696613402099No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53530.23898516354389146No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT52590.23478852514054274No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT52570.23469923496174808No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC51830.23139549834634587No Hit
CCCCTAGACTCCTGATCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCA51260.22885072825069827No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG49300.22010029072882217No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC49200.21965383983484887No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGGAGACTACTGATAGTGAGACTGA48430.2162161679512547No Hit
GGGTGGACATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG48000.21429642910716964No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA47870.2137160429450044No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG47730.2130910116934418No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA46730.2086265027537091No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT46430.2072871500717893No Hit
GATCAGGAGTCTAGGGGCTTTCCCCGGTTTGTGCTGATACCAATTTAAAT46080.20572457194288288No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA44570.19898316344388647No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCAGTCTCACTATC44440.19840277728172123No Hit
GTCTAGGGGCTTTCCCCGGTTTGTGCTGATACCAATTTAAATATCTGACA43360.1935811076268099No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG43170.1927328509282607No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG43150.19264356074946604No Hit
GTGCAGCCACAGTTCCTTTGATGTCCACCCTGGTCCCTCCGCCGAAAGTA43040.19215246476609546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42170.188268341988528No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATGTCCACCCTGGTCC41310.18442886430035788No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA40350.18014293571821446No Hit
GTTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAG40180.17938396919845992No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA40040.17875893794689734No Hit
GCATAGATCAGGAGTCTAGGGGCTTTCCCCGGTTTGTGCTGATACCAATT38520.17197288435850364No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA38010.16969598479923997No Hit
GTCAAGAGACTTATAGTACCCTCAACTTCGGCGAAGGGACCTTTACTTTC37510.16746373032937362No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC37160.16590115220046717No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA36630.16353496246240884No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC36470.1628206410320516No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA36130.16130270799254248No Hit
ATCTGGGACAGATTTCAGTCTCACTATCAGTAGTCTCCAACCTGAAGATT35700.15938296914845743No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC35630.15907045352267615No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35210.1571953597679884No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT34130.15237369011307708No Hit
TGTCCACCCTGGTCCCTCCGCCGAAAGTAAAGGTCCCTTCGCCGAAGTTG33620.1500967905538134No Hit
ATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTTCAGTGGCAGT33550.14978427492803212No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT32420.14473937982613416No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC32310.14424828384276356No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA32070.1431768016972277No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31930.14255177044566514Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG31880.1423285449986785No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA31800.14197138428349987No Hit
TCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTTCAGTGGC31450.14040880615459345No Hit
CTGATAGTGAGACTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGA31430.1403195159757988No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG31220.13938196909845493No Hit
GCACAAACCGGGGAAAGCCCCTAGACTCCTGATCTATGCTGCGTCCAGTC31070.13871229275749503No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA30970.13826584186352175No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT30620.1367032637346153No Hit
GACCAGGGTGGACATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCT30220.13491746015872222No Hit
GATTTCAGTCTCACTATCAGTAGTCTCCAACCTGAAGATTTTGCAACTTA30080.13429242890715964No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG29910.13353346238740507No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA29620.1322387547948826No Hit
ACCTTGATGAGACCCCACTTTGCAGACTGGACGCAGCATAGATCAGGAGT29490.13165836863271735No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28910.12906895344767239No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG28790.12853321237490445No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC28610.12772960076575257No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA28490.12719385969298466No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCAGTCTCACTATCAGTAGTC28480.12714921460358733No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGAGACCCCACTTTGCAGACT28410.12683669897780603No Hit
ATAGTACCCTCAACTTCGGCGAAGGGACCTTTACTTTCGGCGGAGGGACC28370.12665811862021673No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT28220.12598844227925682No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC27950.12478302486552899No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG27770.1239794132563771No Hit
CTATCAGTAGTCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAAGAG27490.12272935075325195No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT27050.12076496681976956No Hit
TCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAAGAGACTTATAGTA27020.12063103155157757No Hit
CTGCTACTCTGGCTCCGAGGTGTCAGATGTGACATCCAGATGACCCAGTC26840.11982741994242568No Hit
ACCCTCAACTTCGGCGAAGGGACCTTTACTTTCGGCGGAGGGACCAGGGT26820.11973812976363103No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA26640.11893451815447914No Hit
CCCTGGTCCCTCCGCCGAAAGTAAAGGTCCCTTCGCCGAAGTTGAGGGTA26420.11795232618773796No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC26180.1168808440422021No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA25990.1160325873436529No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC25360.11321994671162129No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC25080.11196988420849613No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC25010.11165736858271486No Hit
TCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGAT24900.11116627259934425No Hit
ACCCTGGTCCCTCCGCCGAAAGTAAAGGTCCCTTCGCCGAAGTTGAGGGT24850.11094304715235763No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC24680.11018408063260307No Hit
CCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG24610.10987156500682177No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC24590.1097822748280271No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC24370.10880008286128592No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGTC24310.10853221232490196No Hit
TCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTG23880.1066124734808169No Hit
GTTCCTTTGATGTCCACCCTGGTCCCTCCGCCGAAAGTAAAGGTCCCTTC23790.10621066767624095No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT23670.10567492660347304No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT23460.10473737972612916No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23450.10469273463673183No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT23440.1046480895473345No Hit
CTACTGATAGTGAGACTGAAATCTGTCCCAGATCCACTGCCACTGAACCT23420.10455879936853985No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA23160.10339802704420935No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG22750.10156757837891896No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT22650.10112112748494567No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT22630.101031837306151No Hit
CATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG22540.10063003150157508No Hit
CTACAAGACCACACCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT22540.10063003150157508No Hit
CCTTTGATGTCCACCCTGGTCCCTCCGCCGAAAGTAAAGGTCCCTTCGCC22530.10058538641217775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22470.10031751587579378No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)