FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001319203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267245
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT142510.6285602129456676No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC106360.4691156006518925No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC102650.4527521286848135No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT93260.41133622524253No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG80820.3564678718003568No Hit
GACTTATAGTACCCTCAACTTCGGCGAAGGGACCTTTACTTTCGGCGGAG80560.355321105570858No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC80350.35439487130857056No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG74400.328151567210425No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG69340.30583373212864073No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGTCAG69130.30490749786635324No Hit
AGTCAGAACATTGTCAGATATTTAAATTGGTATCAGCACAAACCGGGGAA68270.3011143480303187No Hit
CTATAAGTCTCTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGGAGACT63820.2814870029485124No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA62340.2749592567190577No Hit
CACTATCAGTAGTCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAAG62290.2747387247518464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59740.2634915944240698No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT59560.262697679342109No Hit
GATCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTTCAGTG57440.2533471239323496No Hit
ACTTACTACTGTCAAGAGACTTATAGTACCCTCAACTTCGGCGAAGGGAC56380.2486718462274699No Hit
CTCCTGATCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTT55300.24390835573570566No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54250.23927718442426824No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG52610.23204373589973734No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT51850.22869164999812547No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT51840.22864754360468323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50980.22485439376864874No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG50130.22110535032605652No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA49000.216121327867081No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT48910.21572437032610065No Hit
GGGTGGACATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG48460.21373958262119885No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGGAGACTACTGATAGTGAGACTGA47280.20853502819501202No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC45930.2025806650803067No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCAGTCTCACTATC45170.19922857917869485No Hit
GATCAGGAGTCTAGGGGCTTTCCCCGGTTTGTGCTGATACCAATTTAAAT44860.19786128098198472No Hit
CCCCTAGACTCCTGATCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCA44600.19671451475248594No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATGTCCACCCTGGTCC43660.19256851376891337No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT42980.1895692790148396No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG42820.1888635767197634No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA42690.188290193605014No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT42320.18665825704765035No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG41550.1832620647525962No Hit
GTGCAGCCACAGTTCCTTTGATGTCCACCCTGGTCCCTCCGCCGAAAGTA41300.18215940491653967No Hit
GTCAAGAGACTTATAGTACCCTCAACTTCGGCGAAGGGACCTTTACTTTC39810.17558755229364273No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA39490.17417614770349035No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA39300.1733381262280874No Hit
GTCTAGGGGCTTTCCCCGGTTTGTGCTGATACCAATTTAAATATCTGACA39250.17311759426087608No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA39250.17311759426087608No Hit
GCATAGATCAGGAGTCTAGGGGCTTTCCCCGGTTTGTGCTGATACCAATT38920.17166208327728144No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC38780.1710445937690898No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38170.16835410376911183Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA38160.1683099973756696No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA37850.16694269917895949No Hit
ATCTGGGACAGATTTCAGTCTCACTATCAGTAGTCTCCAACCTGAAGATT36300.16010620819540897No Hit
GTTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAG35900.1583419524577185No Hit
ATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTTCAGTGGCAGT35080.15472522819545306No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35070.15468112180201082No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA34840.1536666747528388No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA33460.14757999245780673No Hit
TGTCCACCCTGGTCCCTCCGCCGAAAGTAAAGGTCCCTTCGCCGAAGTTG33240.14660965180207697No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC32770.14453665131029067No Hit
TCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTTCAGTGGC32700.14422790655619486No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC32340.14264007639227344No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT31680.13972905442508418No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT31500.1389351393431235No Hit
CTGATAGTGAGACTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGA31360.13831764983493183No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG31340.1382294370480473No Hit
GCACAAACCGGGGAAAGCCCCTAGACTCCTGATCTATGCTGCGTCCAGTC31250.13783247950706695No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC30880.13620054294970327No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA30810.13589179819560745No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA30640.135141989507089No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG30500.13452449999889735No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC30340.13381879770382116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29540.13029028622844024No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA29480.13002564786778667No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC29220.12887888163828787No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA29190.1287465624579611No Hit
GATTTCAGTCTCACTATCAGTAGTCTCCAACCTGAAGATTTTGCAACTTA29140.1285260304907498No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC28920.12755568983502003No Hit
GACCAGGGTGGACATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCT28880.12737926426125099No Hit
ATAGTACCCTCAACTTCGGCGAAGGGACCTTTACTTTCGGCGGAGGGACC28720.1266735619661748No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC28530.12583554049077184No Hit
ACCTTGATGAGACCCCACTTTGCAGACTGGACGCAGCATAGATCAGGAGT28240.12455645508094625No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG28180.12429181672029269No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC28150.1241594975399659No Hit
CCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG27890.1230127313104671No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27450.1210720499990076No Hit
CTGCTACTCTGGCTCCGAGGTGTCAGATGTGACATCCAGATGACCCAGTC26850.11842566639247193No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA26760.11802870885149155No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC26700.117764070490838No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT26460.11670551704822373No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCAGTCTCACTATCAGTAGTC26450.11666141065478147No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG26140.11529411245807136No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC26130.11525000606462911No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT26100.11511768688430232No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG25690.1133093247531696No Hit
CTATCAGTAGTCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAAGAG25620.11300057999907376No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA25500.11247130327776662No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25410.11207434573678628No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC25350.11180970737613272No Hit
TCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAAGAGACTTATAGTA25150.11092757950728747No Hit
TCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGAT24920.10991313245811546No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC24660.10876636622861667No Hit
CTACAAGACCACACCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT24560.10832530229419406No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT24170.10660515294994585No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC23910.10545838672044705No Hit
CCCTGGTCCCTCCGCCGAAAGTAAAGGTCCCTTCGCCGAAGTTGAGGGTA23520.10373823737619887No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGAGACCCCACTTTGCAGACT23500.10365002458931434No Hit
ACCCTCAACTTCGGCGAAGGGACCTTTACTTTCGGCGGAGGGACCAGGGT23300.10276789672046911No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGTC23150.10210630081883519No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22970.10131238573687448No Hit
TCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTG22940.10118006655654771No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG22790.10051847065491379No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)