FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001319204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267245
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT150650.6644628172076683No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC118840.5241603796678348No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC116080.5119870150777706No Hit
GACTTATAGTACCCTCAACTTCGGCGAAGGGACCTTTACTTTCGGCGGAG87700.38681307048863267No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT85980.3792267708165637No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG77740.34288310262014027No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC77350.3411629532758921No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG74340.32788692884977144No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT70700.31183220163678826No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA69200.30521624262044905No Hit
CTCCTGATCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTT66880.2949835593418444No Hit
CTATAAGTCTCTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGGAGACT66700.2941896442598837No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC66400.29286645245661586No Hit
AGTCAGAACATTGTCAGATATTTAAATTGGTATCAGCACAAACCGGGGAA64350.28382464180095224No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGTCAG63430.27976685360426423No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG62440.2754003206534803No Hit
CACTATCAGTAGTCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAAG61800.2725775114731756No Hit
GATCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTTCAGTG60110.2651235309814334No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT58120.2563463586864234No Hit
ACTTACTACTGTCAAGAGACTTATAGTACCCTCAACTTCGGCGAAGGGAC57100.25184750655531274No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT55230.24359961098160982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54480.24029163147344024No Hit
CCCCTAGACTCCTGATCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCA52400.23111750163744985No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT52270.23054411852270043No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC51030.22507492573586005No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGGAGACTACTGATAGTGAGACTGA50240.2215905206539214No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG49230.217135774916253No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC48530.2140483273752947No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG48430.21360726344087205No Hit
GGGTGGACATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG47800.2108285606540096No Hit
GATCAGGAGTCTAGGGGCTTTCCCCGGTTTGTGCTGATACCAATTTAAAT47110.2077852195064936No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA46980.20721183639174418No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA46530.2052270486868424No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT46030.20302172901472934No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCAGTCTCACTATC45250.19958143032623296No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA44600.19671451475248594No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG44520.19636166360494783No Hit
GTCTAGGGGCTTTCCCCGGTTTGTGCTGATACCAATTTAAATATCTGACA43210.1905837260640116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42380.1869228954083039No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATGTCCACCCTGGTCC42370.18687878901486166No Hit
GTGCAGCCACAGTTCCTTTGATGTCCACCCTGGTCCCTCCGCCGAAAGTA42000.18524685245749797No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG41980.18515863967061347No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA41810.184408830982095No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA41210.18176244737555933No Hit
GTTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAG39710.17514648835922011No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA39640.17483774360512427No Hit
GCATAGATCAGGAGTCTAGGGGCTTTCCCCGGTTTGTGCTGATACCAATT39450.1739997221297213No Hit
GTCAAGAGACTTATAGTACCCTCAACTTCGGCGAAGGGACCTTTACTTTC38430.16950086999861064No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC37190.16403167721177025No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA37040.1633700813101363No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC36260.15992978262163993No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA36100.15922408032656374No Hit
ATCTGGGACAGATTTCAGTCTCACTATCAGTAGTCTCCAACCTGAAGATT35860.15816552688394947No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC35790.15785678212985363No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34920.15401952590037687No Hit
TGTCCACCCTGGTCCCTCCGCCGAAAGTAAAGGTCCCTTCGCCGAAGTTG34040.15013816327745788No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT33790.14903550344140135No Hit
ATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTTCAGTGGCAGT33590.14815337557255612No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33160.1462568006545389Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA32630.14391916180209904No Hit
TCTATGCTGCGTCCAGTCTGCAAAGTGGGGTCTCATCAAGGTTCAGTGGC32450.14312524672013832No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC32310.14250775721194667No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT32280.1423754380316199No Hit
CTGATAGTGAGACTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGA31970.14100813983490978No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA31910.1407435014742562No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG31220.13770016032674015No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG31220.13770016032674015No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA31170.13747962835952884No Hit
GCACAAACCGGGGAAAGCCCCTAGACTCCTGATCTATGCTGCGTCCAGTC30820.1359359045890497No Hit
GACCAGGGTGGACATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCT30450.13430396803168604No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCAGTCTCACTATCAGTAGTC29720.13108420131040094No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT29710.1310400949169587No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG29510.13015796704811344No Hit
GATTTCAGTCTCACTATCAGTAGTCTCCAACCTGAAGATTTTGCAACTTA29470.12998154147434443No Hit
ATAGTACCCTCAACTTCGGCGAAGGGACCTTTACTTTCGGCGGAGGGACC29460.12993743508090214No Hit
ACCTTGATGAGACCCCACTTTGCAGACTGGACGCAGCATAGATCAGGAGT29070.12821728573665395No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA29050.12812907294976944No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC28990.12786443458911587No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT28740.12676177475305933No Hit
CTATCAGTAGTCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAAGAG28650.12636481721207898No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGAGACCCCACTTTGCAGACT28550.12592375327765637No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG28530.12583554049077184No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA28430.12539447655634922No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG28350.12504162540881114No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28210.12442413590061946No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT28080.12385075278587007No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC27370.1207191988514695No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA27230.12010170934327787No Hit
CTGCTACTCTGGCTCCGAGGTGTCAGATGTGACATCCAGATGACCCAGTC27190.1199252837695088No Hit
TCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAAGAGACTTATAGTA26930.11877851754001001No Hit
ACCCTCAACTTCGGCGAAGGGACCTTTACTTTCGGCGGAGGGACCAGGGT26720.11785228327772251No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC26140.11529411245807136No Hit
CCCTGGTCCCTCCGCCGAAAGTAAAGGTCCCTTCGCCGAAGTTGAGGGTA26050.114897154917091No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC25650.11313289917940055No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC25640.11308879278595829No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC25560.1127359416384202No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA25370.11189792016301724No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC24840.10956028131057736No Hit
TCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGAT24830.10951617491713511No Hit
CCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG24710.10898689819582798No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC24560.10832530229419406No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC24410.10766370639256015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24280.10709032327781073No Hit
ACCCTGGTCCCTCCGCCGAAAGTAAAGGTCCCTTCGCCGAAGTTGAGGGT24170.10660515294994585No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT24120.10638462098273456No Hit
TCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTG24080.1062081954089655No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA23890.10537017393356254No Hit
CTACTGATAGTGAGACTGAAATCTGTCCCAGATCCACTGCCACTGAACCT23850.10519374835979349No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT23470.10351770540898755No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGTC23340.10294432229423817No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG23330.1029002159007959No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG23110.10192987524506615No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23050.10166523688441259No Hit
GTTCCTTTGATGTCCACCCTGGTCCCTCCGCCGAAAGTAAAGGTCCCTTC23000.10144470491720127No Hit
CCTTTGATGTCCACCCTGGTCCCTCCGCCGAAAGTAAAGGTCCCTTCGCC22940.10118006655654771No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT22910.10104774737622092No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT22710.1001656195073757No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT22680.10003330032704891No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)