FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001319493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA10.628930817610063No Hit
AGTTTGAGTTTGATGCTCACCCTGATCAGAGGATTGAGTAAACGGCTAGG10.628930817610063No Hit
GAGTGGCATTTTTTTGACATCGATTATCTGATAACGAAAACCGCTATAGA10.628930817610063No Hit
GTCTACAATGTGTAAAATGAGCCCAGGTCTTTCTTTTCCGCCCACCCCTT10.628930817610063No Hit
GATCTACATATCCAATATCCACGTATATTTTAGCAAACAGAGATCCCAGT10.628930817610063No Hit
GGATTACAGGCGCCCGCCACCACGCCCCGTATTTTGCGTATTTTTCGTAG10.628930817610063No Hit
GTATATGACTTTATGTCCGAAGGATCTTTTCAAGTTTGTGGTGATCAATA10.628930817610063No Hit
TTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGT10.628930817610063No Hit
CCCACAGGCCGGTGGCATCGGCGCGGAGTGCCCAGGTTCACAGTGCAGCT10.628930817610063No Hit
TTCTCATAGGAGAAGATTAATTTGTAAATAAGAGATACAGTACTTGTGAT10.628930817610063No Hit
CTTAGAGCACTATTTCACAGCTTTCACATAGCACAGATAACTCGCCATCT10.628930817610063No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC10.628930817610063No Hit
TGACCAGGCTGGTCTCAAACTCCTGATCTCAAGTGATCCTCCCACCTCAG10.628930817610063No Hit
ACCGAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA10.628930817610063No Hit
CTATAGCGGTTTTCGTTACCAGATAATCGATGTCAAAAAAAATGCCACTC10.628930817610063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
GAGGGTTTTCTCACCTAAATGATGGTCGGGCACCAATAAAGTGCCATCCA10.628930817610063No Hit
GTACTTGGGGGCTGAGGCGGGAGAATTGCTTGAACCTGGGAGGCGGAGGT10.628930817610063No Hit
GAATAGCAATGAACCTATAAATTACTTTGGGTAGTATGGCCATTTTCATG10.628930817610063No Hit
CCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAG10.628930817610063No Hit
CCACTTATGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAA10.628930817610063No Hit
CTGCTTCTGTGGCGATCACGACGATCCTACTCGCTTGCAGATCCAGCTAT10.628930817610063No Hit
CTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTC10.628930817610063No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT10.628930817610063No Hit
ACTTTATATTTTGTATTATGTCAAATGTTATGGCAGGGCCCAAATAGCCT10.628930817610063No Hit
CATGAAAAGTGGAAAAGTTTAGATCTATGGAAACAGAACTGTTGCCTATA10.628930817610063No Hit
TTCTGACTCAACAAATGGGGAGAGCAAATTGAAAATGCGTAAATTGGAAG10.628930817610063No Hit
GCGTTGATACCACTGCTTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT10.628930817610063Clontech SMART CDS Primer II A (95% over 21bp)
GATCTGACTTAAGTTTTGTTTTGTTTTGTTTTTTGAGATAGAGTCTCACT10.628930817610063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10.628930817610063No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC10.628930817610063No Hit
GTGTCTGATCCGTCCTTCCTTCTCCGGGTTTGGCAGCTGAGGCAGCGTGG10.628930817610063No Hit
ATGCAGGTTAGTTTAAACTTTGAAGGAAATTTTTAAGGTGGCAAAAGGTT10.628930817610063No Hit
NGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATC10.628930817610063No Hit
GGTTTCCTCTCCTGTAGCATAATCCACTGAGTATGCAGGTGTCCTGTGGC10.628930817610063No Hit
GATTAGGAAGAGGGAGGCCTGAGAAAGCAAGCATCATTTCATAGGGTGGA10.628930817610063No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT10.628930817610063No Hit
AAAGAAAGACTCCCGAATGCGGCGCAGAACATTGGGGATTCCTTTAGTGA10.628930817610063No Hit
TATCAACGCAGAGTACATGGACTAGCCGGGAGACCAGAGATCTAGCGACT10.628930817610063No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCCCACTTTTGCCA10.628930817610063No Hit
GAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAAAAA10.628930817610063Illumina PCR Primer Index 1 (96% over 27bp)
GCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGAT10.628930817610063No Hit
TCCATGTGCTGATGGGTGATGTGACTGTGGTCAGCAGGCTTGGGAAGTGC10.628930817610063No Hit
CAATTAGGGCTTCCTGGGAACTGGCAGTATGAGGCATTTTAGTCACTGAG10.628930817610063No Hit
TCCTTTCACATAAGATTTCTCTGTAGTATGCAATGCTATTTGATAGCATT10.628930817610063No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA10.628930817610063No Hit
GCATGGCTACAGTGCGCCCTGGTTGTGCCACTGCACTCTAACCTGGGCAA10.628930817610063No Hit
CCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTAT10.628930817610063No Hit
ATTTTATAGGATTAAGATTTTGAACATAGGATGATGACATGATTTGACAA10.628930817610063No Hit
TTTTTGTGGTCTATTTAGTGCCATATTTTTCACATTGTTGTGCTTTCTGT10.628930817610063No Hit
CAACTTGGTTATTGTAAAAAAAGGAGAGAAGGATATTCCTGGACTGACTG10.628930817610063No Hit
AATAAGCACTGGTGGATATTTATTTTATGCAATAGGTTATAATTCATTAC10.628930817610063No Hit
GGATAATTGGACAGTCATGAGTGGATCATCGAAGGGATAAGCAAAATATA10.628930817610063No Hit
GACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTCCTGG10.628930817610063No Hit
GCCCTTGTTATTCCTGATTATACCTAGTGTATAATTAAACGCTGAACTGA10.628930817610063No Hit
AATTAGAATTGTGAAGATGATAAGTGTAGAGGGAAGGTTAATGGTTGATA10.628930817610063No Hit
GTTCCAAAAGTAGATGATGAAATCCTAGGGTTTATTTCTGAAGCCACTCC10.628930817610063No Hit
GCCTGGCTGTGGTAGTTTCTTCCCACTTAAAAATTCATAGAGACAACTCT10.628930817610063No Hit
GTCTTGCTCTGTCGCCTGGGCTGGAGTGCGGTGGCGTGATCTCGCCTCAC10.628930817610063No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA10.628930817610063No Hit
GATATATAGATATCCAGTGCATGCGACTTCATATTTTCTAATAGTGGTAG10.628930817610063No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
TCAAATACGCATCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGC10.628930817610063No Hit
ACATATAGAAGGGCATTATAGATATGATGTCCATCCCTATGAAGCTTACA10.628930817610063No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG10.628930817610063No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCG10.628930817610063No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC10.628930817610063No Hit
GTTTAGGGCCTTTGCACTAGCAATTTCTTTTGTTTGAAACACTACCACCA10.628930817610063No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10.628930817610063Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTACC10.628930817610063No Hit
GAAGAGGAGAGAACTCCTGACCTCAAGTGATCCGCCTGGCCAACATGGTA10.628930817610063No Hit
GTATAGGTGAGGGACTTGATCAGGCTCTGCCCTGTCTGACAGAACTCATT10.628930817610063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT10.628930817610063No Hit
GCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATG10.628930817610063No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT10.628930817610063RNA PCR Primer, Index 31 (95% over 24bp)
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC10.628930817610063No Hit
GAGAGGGGAGGTATCTGCAGAGAGGGGAAGTCACCCTGCAGGTAGGAAGC10.628930817610063No Hit
CCTCCCATCTTCTAGAACTGAGATGTTCTGTGTTCAGAGAAATTCCCAGC10.628930817610063No Hit
GCTTACTTCTCTTCCTTCTCTCCTTAATGACATTAACACTATTCTCACCA10.628930817610063No Hit
ATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATC10.628930817610063No Hit
TCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATA10.628930817610063No Hit
GTACAGTATTTGTCTTTTTGTAACTGATTATTTCATTTGGCATAATGTCC10.628930817610063No Hit
GTTAGTTCTTCTGCTGTTAGGAAGCCACTATGTCTGGACGTGGAAAGCAA10.628930817610063No Hit
GCCGTAGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTA10.628930817610063No Hit
TCCTAGTGTCTGGTGGTTGCCAGCAATCCTTGGTGCTCCTTGGCTTGTAG10.628930817610063No Hit
TATCAACGCAGAGTACATGGGACCGTTAACTTCCAATTAACTAGTTTTGA10.628930817610063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
CCAAAGTAGAGAGGGTTTTCTCACCTAAATGATGGTCGGTCACCAATAAA10.628930817610063No Hit
TCCTACACTCGACCCCTCGAAACCAGTCCTTACAATCTTACATGCCTGCT10.628930817610063No Hit
ATTATGTATTCCTCTTTAGATTTTTAAATAAAGAAAAAAAAATCCATGCC10.628930817610063No Hit
AACGCAGAGTACGTGGGTAGCAATAAACGAAAGTTTAACTAAGCTATACT10.628930817610063No Hit
ATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCAGGGAT10.628930817610063No Hit
ATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCAT10.628930817610063No Hit
CCTTATAGATGCTTTAGGGTAAACAAAAAGTCTAAGTTATAGTCTGCTTT10.628930817610063No Hit
GTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAATA10.628930817610063No Hit
ATGTAATGGATTTGTTAAAGTCAAATCAAAGAATATGGAAATTTATGCTA10.628930817610063No Hit
CACCGTGCCTGGCGATAAATTTGACCTTAGTATGTTTATTTCGGAGCTTG10.628930817610063No Hit
GGGCAACAGAATGAGACCCCGTCACCACTAAAAAAAAAAATTAAGCCAAG10.628930817610063No Hit
GCGTTGATACCACTGCTTGGTATCAACGCAGAGTACATGGGGGGAATTCG10.628930817610063No Hit
ATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATC10.628930817610063No Hit
TTCTTATGCTTCTTTTACAACTACATCCCATTTTATATTTCCAATTGTTA10.628930817610063No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG10.628930817610063No Hit
GATTTCTGCGGAGATATGAGCTCCTGATATTAATGAGGGTCTCTTGTAAA10.628930817610063No Hit
AAATAGTATGGCTTTGAAGAAGGCGTGGGTACAGATGTGCAGGAATGCTA10.628930817610063No Hit
GTATCAACGCAGAGTACATGGGAGCTGAGCCAGTTCCAGCTGTTGGAACA10.628930817610063No Hit
CATGAGGAGAGGGAACATGCTGAGAAACTGATGAAGCTGCAGAACCAACG10.628930817610063No Hit
CCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGA10.628930817610063No Hit
GTGTACAGAACTCCCCTATAAAGAAAACATGTGGAGTGCTTAATTCCTTC10.628930817610063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTA10.628930817610063No Hit
GTATCAACGCAGAGTACATGGGAGGAGAATCACCTGAACCCAGGAGGCAG10.628930817610063No Hit
TTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACG10.628930817610063No Hit
CAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATG10.628930817610063No Hit
ACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTACTACCAC10.628930817610063No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT10.628930817610063No Hit
CTATGGCAGCCATTGATGCTGTGTCACCCACACGGTTGGGCTTTAAGAGA10.628930817610063No Hit
AGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACG10.628930817610063No Hit
GAGCAGCTGCTGATGTCTGGAGGCTGATTTTCCTGTTCTCTGTTCTCCAC10.628930817610063No Hit
GTAATAAGTGTGCAAGGTACTAAATAGAGGAGAGCAGGATGCCCCTTTTT10.628930817610063No Hit
GAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAA10.628930817610063Illumina PCR Primer Index 11 (96% over 29bp)
GGAACAAACATCACAACTATGCCAAATAATCAATCCTACAATGTCAAAAA10.628930817610063No Hit
GATCTGACACCTATAATTTTTATTACTTAATCTCTTTCTCCTTCCCTTTA10.628930817610063No Hit
GTTTCACTACGTTGGTCAGGCTGGTCTCGAACTCCTGACCTCGTGATCCA10.628930817610063No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA10.628930817610063No Hit
CACATGAACAATGAAACCGATTTATTTTTCTGGAAAGGCACATTGAGCTG10.628930817610063No Hit
CACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCT10.628930817610063No Hit
ACCTCTGTCTCTTGGGTTCAAGAGATTCTTCTGCCTCAACCTGCCGAGTA10.628930817610063No Hit
AGGGAAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATAGCAGATG10.628930817610063No Hit
ATGGTGTATAGAAGCTGGCTCCTACTGGCTCATAAAAATTATTGTTAAAT10.628930817610063No Hit
GCTTTTAGGCCCACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTT10.628930817610063No Hit
TATCTGGACATTTTTTGGTTTTGTTCTGTTTTGTTAAAAAAAAGAAAAAG10.628930817610063No Hit
CAATTAGTGTCCAATACTTACAAACTTTTTTTTTCTTTATTCGAGACAGA10.628930817610063No Hit
GATATATACCCAGTTATGAGATTGCTGGGTTGTATGACAGTTCTGTTTTA10.628930817610063No Hit
GCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGA10.628930817610063No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC10.628930817610063No Hit
ACGCAGAGTACATGGGGCTCTACCGGCGGGATTTGATGGCGTGATGTCTC10.628930817610063No Hit
GTATCAACGCAGGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA10.628930817610063No Hit
GCAGAGTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGAT10.628930817610063No Hit
CAATTACACAAAGCATTAAAATTTACAAGTATGATCTAACCAATTATCAA10.628930817610063No Hit
TCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGAAATGAATGA10.628930817610063No Hit
GGGTGCACATGCTGCGAGGTCTCCATCCTCCGGGCCTGTCCCTCCCAGAT10.628930817610063No Hit
TCTCCGAGCCCAGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA10.628930817610063Illumina PCR Primer Index 11 (96% over 29bp)
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT10.628930817610063No Hit
GATCCAGGAAGCACCAAGAACACCAAGCAGAATAAATATCAAAATGTCTA10.628930817610063No Hit
ACATTGACGTCACCATCAACATCATCATCTTCCTCCTCTTAGCAGCATTG10.628930817610063No Hit
GGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATAT10.628930817610063No Hit
TGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCG10.628930817610063No Hit
CTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA10.628930817610063No Hit
GTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAAC10.628930817610063No Hit
ACTGTAGACATGAGTCTTGCAAATGCATCAGTCACTTTGAGGCTTGAGGT10.628930817610063No Hit
TCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCAT10.628930817610063No Hit
CTTGGAACACTAGAACTAATCAAATCATCTTGTCTTCTACCAAAGGAGCA10.628930817610063No Hit
ATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGC10.628930817610063No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA10.628930817610063No Hit
GGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCT10.628930817610063No Hit
GCTGCCCCCGGCCGGGGGCAGCTGGAGGTGCCTCAGAAGGTGCATTCTGC10.628930817610063No Hit
CTCTTTCATCGACCTCCCCACCCCATCCAACATCTCCGCATGATGAAACT10.628930817610063No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC10.628930817610063No Hit
GTAGCTGGGATTACAGGCGCCCACCACCACACCCAGCTAATTTTTGTATT10.628930817610063No Hit
TGCTAGGGTGGCGCTTCCAATTAGGTGCATGAGTAGGTGGCCTGCAGTAA10.628930817610063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)