FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001319494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTTGTGATAAGGGGTTAAGTATCCAAAATCTATAAGGAACTCACCAGC10.628930817610063No Hit
GTATCAACGCAGAATACACAAGCATAGAGCCAAATCATGAATGAACTCCC10.628930817610063No Hit
CCGGCAGCACGTCGAGGCAATCCGTGGCGGAGAAACACAAATGTGCACGC10.628930817610063No Hit
GTATCACCGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCT10.628930817610063No Hit
GAATCATTCCGCTATTTGCGCTTAGATAGGTTTCGCTACCATGGGACTCA10.628930817610063No Hit
ATATGGTCAGGAAAACATAAAATGCGCCCATAAAAAAATTAAGAACAAGT10.628930817610063No Hit
CAGTTCCAGGTCTAAGGCGCAAAGTTCCCTTTTAGGGGAAGAAACAACTT10.628930817610063No Hit
TAATTATGCTTCATAGGGATAGTACAAGGAAGGGGTAGGCTGTGTGTTTT10.628930817610063No Hit
ATTCAACAAAGCCTGCAAATCTCACTCTTGTGGAATTCCACTGGACCCAA10.628930817610063No Hit
GACTGAATGTTTTGGGCGCACCGTCGACGCAGCGCCGTTTTTGCATGCCA10.628930817610063No Hit
GATTTATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTCCCGCAC10.628930817610063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
GCGAAGGCTGTATATATTGAACATTATTTTTGTTGTTCTATTATAAAGTG10.628930817610063No Hit
CGACATTACCCTCACTTTTGCCACGGGGCGGCATGCGCTGGAGATGCAGC10.628930817610063No Hit
GATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCTACGG10.628930817610063No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG10.628930817610063No Hit
GGATAGCACTGTATTTGTGCCCGACCATCATCTAGGTAAGAAAACCCTCT10.628930817610063No Hit
GCCACTGCTAGTGGATATGGAAATTGGTACAACTTTTCCAGATGATAAAG10.628930817610063No Hit
ACATGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGAT10.628930817610063No Hit
TCGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGAGCCTTAGAGCCGTGCC10.628930817610063No Hit
GTACTTGGTAAACGCCCTCTTCTTAGATTTATGCCGATTCTTATAGAAAC10.628930817610063No Hit
CCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCT10.628930817610063No Hit
CCTTTTGTTTCCTGTTTTTTGGAATTTTTTTTTTTAGTTGACTTCAACTT10.628930817610063No Hit
GTAATTTATAGATTCAATGTTATCCCCATTAAGCTACCATTGACTTTCCT10.628930817610063No Hit
GTCCAACTGCTGATTGCAAGACTCAGTGGAGGCTGCTTGAATACCTCCTA10.628930817610063No Hit
TTGTTGAGATGTCAACAAAGACTTATAATTTCCTACAAACTTAGTTGATA10.628930817610063No Hit
ATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCAT10.628930817610063No Hit
GGACAGCACTTTATTGGTACCCCACCATCATTTAGGCGAGAAAACCCTCT10.628930817610063No Hit
GTCTTGGAACAATAAGCAAACACTGCTTAAATTTTTCATTCAAATTCACT10.628930817610063No Hit
GTATATGGACCTGGTCTCAAAAGGAAGTGGGGCAATTCTGGGGTTCTAAA10.628930817610063No Hit
GACAAACCTTTCGCTCTCGTTCAAAGCATCACCACCCCACGTGCAATCGC10.628930817610063No Hit
ACATTACCCCCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT10.628930817610063No Hit
GCATATTCTCGGGGCGCTCTCGCTGGTTGCGTATTTGATTACCGGCAACG10.628930817610063No Hit
ATATTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGCA10.628930817610063No Hit
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGT10.628930817610063No Hit
CAGGGAAACAAAAGAAATATACAATCTGGGACAACTGTTCTTTGGCAAGG10.628930817610063No Hit
ATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAAC10.628930817610063No Hit
GGAGTACGTGGGCCTGTCCCAGCAAGGGCTCTGGCAGCTGACAGGGCTTT10.628930817610063No Hit
CACTCCTGTAATCCCAGCACTTTGGGAAGCCGAGCCGGGCTGATCACCGA10.628930817610063No Hit
CGGATAAGGCATCGCTACCCCTTCCTGTCACTCATGACACCACTGCACAC10.628930817610063No Hit
GTGCTGACCCAACATTTAGGTTTATCGCTGCGCGATTGCACGGCCTTTGG10.628930817610063No Hit
GCGTCACTGGCGTTGAGAAGGGAAATGAAATGGCTCTCACATTGGTCATA10.628930817610063No Hit
TCTTTATTTAAGGGCTTTCTTACAACATACTGGCGGACATCATCTTCTTT10.628930817610063No Hit
CTATCGACGCTGACTAACATGTCGGGATCGTTGATCTCATCACCGAAGGC10.628930817610063No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTCACTCC10.628930817610063No Hit
GCATTGTGAAACACTGTCTCTACTAAAAATACAACAATTAGCCTGGTGTG10.628930817610063No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGACAGCA10.628930817610063No Hit
TTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCA10.628930817610063No Hit
TTGGAGGTACGCAGGTAGGATCTAAAGCGACATGAGCCGCCCACGGGTCA10.628930817610063No Hit
TCCCCACACCTCCCTACTCCCCTGGGCATCTTCTGGCTTGACTGGATGGA10.628930817610063No Hit
GTCAGATATTCCAGCACCGCTGCCAGGTACACTGGAGCGCCGGCCCCGAC10.628930817610063No Hit
CATTAGGCGAGCGTGCGCATTTGTGTTTTTCCGCCACGGATTGCCTCGAA10.628930817610063No Hit
TTGGGAGGCCAAGGCGGGTGGATCACCTGCGGTCAGGAGTTCAAGGCCAG10.628930817610063No Hit
CCTCTCTTGTTAGGCCCATTCTATTGATTATTTGAGGTCCAATTCAAAAC10.628930817610063No Hit
GCATAGGAGCCCTCTGGCTCCTTTCAAGATTTTCTCTTCTTTTTTGGTTT10.628930817610063No Hit
TTATAGATTGGTCCAATTGGGCGTGAGGAGTTCAGTTATATGTTTGGGAT10.628930817610063No Hit
CAATTAAAATGCTACATGGGAATGCAGAATATCAGATATTTTATTGTCAT10.628930817610063No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT10.628930817610063No Hit
TTACAGAATATTGCCTTTTGCATCTTGGATTGTGGCTAACTGGGTAACCC10.628930817610063No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA10.628930817610063No Hit
TCCAATGAGAAGTAGTAGTGCGGTCGCGCGACGAAAAAACAAAATGTTGG10.628930817610063No Hit
ACGCAGAGTACATGGGATTCAATGGAACATCACCTGAGATAGTAGAAAAA10.628930817610063No Hit
GGAGGAGATAGCCACTGGCCAGGCATTCCCCTTCACAACAGTAGAGACCT10.628930817610063No Hit
CTATAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCCCCCCCTAGCA10.628930817610063No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG10.628930817610063No Hit
ATGTCTGGGGTTTGGTTAGAATTTTATGCCGACATAAACCAAACTTGTGG10.628930817610063No Hit
GTGGTATCAACGCCAAGTACTTTTTTTTTTTTTTTTTTTTTTTTTCTTTT10.628930817610063No Hit
GTATAACTCTCCGCCAGCACGTTTTTCGGTTTCATCTCTTTATCCAGACC10.628930817610063No Hit
CTATAAAAACCAACCTTGTTAGAACTCTGGAAAATAATTAAAGGTTTACA10.628930817610063No Hit
CCCCAGCCTAGAGGAATCCACGCCGCGCCCCAGTCGGCCGTGCTAGCGTG10.628930817610063No Hit
CTTTAATACTACAGCATAGTTCCATCATAAAGGAAAGGAGAAAGAGTTGA10.628930817610063No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT10.628930817610063No Hit
GATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCG10.628930817610063No Hit
CTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTC10.628930817610063No Hit
GTGCCCGGCCTTGAAACTGGCTTCTTTCACTTTGCCATAATGTTTTCAAG10.628930817610063No Hit
GAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAAGGCACCTTATTTG10.628930817610063No Hit
CTCCATTGCATTCAGCCCGCTCTCCCAGTCATCACAGTCTGGTTTCTTGA10.628930817610063No Hit
TTGTGATTCTTTGACTGTGACACTATTGGATGTGATTCTAAAAGCTTTTA10.628930817610063No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTTTTTAAAAAAA10.628930817610063No Hit
AGGGAGGCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCGGAGGTTGCA10.628930817610063No Hit
NTACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGA10.628930817610063No Hit
GATATATTCTATTTACATTTATTTTTTATCTAAAGAAACAAAATGAATTA10.628930817610063No Hit
ACCTAGGTCTGTGATCATCACGCTCTAGACGCCGCGGTTTTTGCCGCCCA10.628930817610063No Hit
AAAAGATACTGCAGGTGTTACACGTTCAGCTTCAAGTCCCAGACTTTCCT10.628930817610063No Hit
GGCAGATAAAGAATATTGAGGCGACATTGGCGTGAAGGTAGCGGATGATT10.628930817610063No Hit
ATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAAC10.628930817610063No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA10.628930817610063No Hit
GGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAG10.628930817610063No Hit
AAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTCGGGTATCCCATT10.628930817610063No Hit
GAATTTGAGACTAGCCTGGGCAAAATGACGAGACCCCATCCCTAAAATTA10.628930817610063No Hit
AACACAAATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAG10.628930817610063No Hit
GCCGATAATAAATAAAATAATTACACGGAGTTATTTTAAACAGAGGTTCA10.628930817610063No Hit
GCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGAT10.628930817610063No Hit
AGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCTACAG10.628930817610063No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.628930817610063No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10.628930817610063Clontech Universal Primer Mix Long (96% over 25bp)
GGCCAAGCTGAAGAAAACGGAGACGCAGGAGAAGAACACCCTGCCGACCA10.628930817610063No Hit
TCATTTGACAAGGCGGTGGATGAATTTTATTCCAAGATAGAAGGCCAGAA10.628930817610063No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC10.628930817610063No Hit
GTGATAATATAGTGATTCATCCTGGTTGGGAATGTTGTGTTTTATTATAT10.628930817610063No Hit
CCACTGAAGCGCCATCCATATCAAATGCTGCCAGACGAGCCATTTCTTTC10.628930817610063No Hit
ACTCAACACACACATTATTCACATCCTATTGGTATACTGGTATGTTGACT10.628930817610063No Hit
GTATCAACGCAGAGTACAATGGGATACAAGCAGCCAACAAACATACTAAA10.628930817610063No Hit
CACCTAAATGATGGTCGGGCACCAATAAAGTGCCATCCATATCAAATGCT10.628930817610063No Hit
CTATTGGCATGATACTTACATTTGTTGTTCTGGTGCTGATGAAAAAGGGG10.628930817610063No Hit
GTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTGCTGACCCA10.628930817610063No Hit
CAGTCTGCCTGCTTTCTGATTGTATCCATTGAAGTGTATGTACATTATGG10.628930817610063No Hit
CTTTATTGCTGCCTACCGTGCGCACTCCGAGGCGATACGGGGTGGAAACA10.628930817610063No Hit
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT10.628930817610063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC10.628930817610063No Hit
ATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTG10.628930817610063No Hit
CGGTAGTTGCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCGGC10.628930817610063No Hit
GCCTGGTCCAGTGGGTGATCAGTGCCAATCTCATTGGAAATGTGGCATTA10.628930817610063No Hit
GTAAAAGTTCACCTTCCAGAGAATGCGCGCGCGTTCCGTTGCCGGTAATC10.628930817610063No Hit
GACTAATATTTCGCGATCGTTCATCGCATCACCAAAAGCCGTGCAATCGC10.628930817610063No Hit
CCCTAAACCTCAACAGGTACATCAACAAAACTGCACGCCAGAACACTACG10.628930817610063No Hit
ATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTG10.628930817610063No Hit
TCACTTCATCACAGTACGGTCAGTTGCCCAATAAGTTCCATCCAGTAGTC10.628930817610063No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC10.628930817610063No Hit
ATCTGGGAGGGACAGGCCCGGAGGATGGAGACCTCGCAGCATGTGCACCC10.628930817610063No Hit
TATCAACGCAGAGTACATGGGATCCTTGGAAAGTGATGAAGATGTGGAAG10.628930817610063No Hit
CAGCGAGAGCCATTTACAATTGCACAAGGTAAGTTTATTCTCCCTTCCTT10.628930817610063No Hit
GGATGACGGTGGCCCCTAATCCACTGGCTGGTGTCCTGAGGACAGAGACA10.628930817610063No Hit
CATTGAAGATGTGCATGCTGGCTCGGGCAACCAATTGCCGATACAGCACC10.628930817610063No Hit
GTATCAACGCAGAGTACATGGGGGAGGGCGCGGCGGATAGGGATCAGTGG10.628930817610063No Hit
GTTCTATGTGAATTTCATCCTGTCATCATGGTCCTAGCTGGTTATTTTGC10.628930817610063No Hit
TTACTCTGTTGCCCAGGCTGGAGTGCAGTGGCGCGATCTCGACTCACTGC10.628930817610063No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG10.628930817610063No Hit
GTGTTTCCCCTAAGACAGCAAATCGCCAATTCATATGTCTATCCTAGAAA10.628930817610063No Hit
CTAAAATTATCTTGTGGAGGAAAGAAAAGGAACAAATGGCAACCCCATCT10.628930817610063No Hit
AGATAAGACTGTTCAACCTGTTCCTGCTCCGGACTCCACTATAGCAGATG10.628930817610063No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT10.628930817610063No Hit
GAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAATGCTT10.628930817610063No Hit
GATAGTAATAGGGCAAGGACGCCTCCTAGTTAGTTAGGGACGGATCGGAG10.628930817610063No Hit
GGTCGGGAGTGGGGAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10.628930817610063No Hit
CACTAAAGGAAGTGCAATCGCGCAACGATAAACCTAAATGTTGGTTGAGC10.628930817610063No Hit
TCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTAT10.628930817610063No Hit
CTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAG10.628930817610063No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC10.628930817610063No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCGGAAAATTGGGG10.628930817610063No Hit
TCTCCTGCCCACAGTCTGTTCCTACTATCGCACCTCACTCCTTTTTCCCC10.628930817610063No Hit
CCTTAGCTCTCACCATCGCTCTTCTACTATGAACCCCCTTCCCCATACCC10.628930817610063No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC10.628930817610063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
CCTTTTACCTATTTCTGTGGTGTAAATATGCCCTTCATGGGCAATTTCAA10.628930817610063No Hit
CCGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC10.628930817610063No Hit
GTACATGGACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACC10.628930817610063No Hit
GCAATGGGCCACGAGTTTGGGAATCTGACGCGGATGCGGCATGTGATCAG10.628930817610063No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG10.628930817610063No Hit
CCCCAGGACCCTGAGGCACACTCAGCTCCCGCGCCTGCTTCTCTGTCACC10.628930817610063No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT10.628930817610063No Hit
ATCTAAATGTTACTAGATCAAATTTATAATGCAACTGGAACCTAAATGAC10.628930817610063No Hit
GAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTT10.628930817610063No Hit
TTCTTGGTGTGGCTTGGGGGTTTTTGGCTTCCTGTTTTATCTCGTGTGTG10.628930817610063No Hit
GGTATCAACGCACAGTACATGGAGGGCCGGGCGCAGTGGCTCACGCCTAT10.628930817610063No Hit
GGGTATATTGGTGTTCTTGTATAAAAATTCTTAACAAATACAGAAAGGCA10.628930817610063No Hit
GTACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCCCCTTTTTTT10.628930817610063No Hit
GGCATGATAATGGCTCACCGCAGCCTTGATGTCCCAAGCTCAAGAAATCT10.628930817610063No Hit
GCGTATGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGAATTGC10.628930817610063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)