FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001319495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCTCTGAGAGTAATTCTTTTGGCTGGATCATACTCCAACATTTTCTG10.6711409395973155No Hit
AAAAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCTGTTGT10.6711409395973155No Hit
GTACATGGGGAGGTGATCTGCCCGCTTCAGCATCCCAAAGTGCTGGGATT10.6711409395973155No Hit
ACTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACAC10.6711409395973155No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG10.6711409395973155No Hit
GTCCCTGGCTGACAAGCATGCTTACAAGAGAAAAAGGCAGAGCTCTCTTC10.6711409395973155No Hit
GTCCCACCTACTCAGGAGGCTGAGGTGGGAGGATAGCTTGAGCCTAGGAA10.6711409395973155No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10.6711409395973155No Hit
CCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT10.6711409395973155No Hit
GTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCT10.6711409395973155No Hit
GCAATTTATTGGTCACATATACTCAAAGCCTGGAAGAGGAAGGCATCACA10.6711409395973155No Hit
GCATATTCCAATTACATGTATATTGTGACTGCAGTGAGTTGGAAAATAAT10.6711409395973155No Hit
GTCTTTATCTGCCCCTTTAACAAGTTGAGTAAAAATAAAAGGTATTTTTT10.6711409395973155No Hit
TCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCAT10.6711409395973155No Hit
GGCCAACATGGTGAAACTCCATCTCTACTAAAAAAAAAAAAAAAAAAAAA10.6711409395973155No Hit
CTTTTATTTTGGTGGGGGAAGGGGCAGGATCTGTAAATATGCTGCTTCAT10.6711409395973155No Hit
CCTTTGTGGTTTGGTGGCGTCCATAAGTTTAGGTCCTCTCCATTATTAAC10.6711409395973155No Hit
CTTCTCTCCTTAATGACATTAACACTATTCTCACCAGACCTCCTAGGCGA10.6711409395973155No Hit
GACTTGTCCATCCCCAACGTACATTCAAGTCCTATGCCAGCAACTTTGTC10.6711409395973155No Hit
GTATAATACCTGATTGCAGAAGTGGATTTGAGATGAAGCTTTCTCATGCT10.6711409395973155No Hit
CTCCTGATGCGAGTAATACGGATGTGTTTAGGAGTGGGACTTCTAGGGGA10.6711409395973155No Hit
GTCCTGGGCTTAAGTAATCCTCTCACCTCAGCCCCCCAAAGTGGTGGGAT10.6711409395973155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTCCCCCGGTT10.6711409395973155No Hit
CGTACTAAATGTCTTAGTGCCTCCTTGTCTGAATGTTGCATCAATCACAG10.6711409395973155No Hit
TTCCTGGACCTCCAAAGCATTTTTTTTGAGACACTGCTTCACTCTGTCAC10.6711409395973155No Hit
ATTGCACGGCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGC10.6711409395973155No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA10.6711409395973155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAGT10.6711409395973155No Hit
CTAGGGGACCACGGTACAGCGATAAGTCACTCAATTACTACCTTATGATT10.6711409395973155No Hit
CAACAAATAGTGTTGGGGTAATTGGCAAGCTACGTGTAGAGGAATGAAAT10.6711409395973155No Hit
TAATTAGTCAAGCCAAGAAAACAGGAAGTAGAAATAAGCATTCTTTACCT10.6711409395973155No Hit
CCCATCATCCGCCTTCTGGTAGAGTGTCTCCTGGAAGAGGATGACACCCC10.6711409395973155No Hit
ATATATTGCTTGTTACCATATTCCCAGCACCTACAATCATGTGTGGGAAA10.6711409395973155No Hit
CCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGG10.6711409395973155No Hit
CTGTTGTCCAGGCTGGAGTGCAGTTGCATGATCACAGTTCACTGCAGCCT10.6711409395973155No Hit
GGATGAAAACGCAAAGCCCTATCACTCATTTCTCCTTCACTTGCCATCAG10.6711409395973155No Hit
AAGTAGGAGAGTGATATTTGATCAGGAGAACGTGGTTACTAGCACAGAGA10.6711409395973155No Hit
GCCACAGAACTAATCATATTTTATATCTTCTTCGAAACCACACTTATCCC10.6711409395973155No Hit
TGTCACTAGATTGCAGCGTGTCCACTAGGGAGCCTTCTTGGACCATCTTT10.6711409395973155No Hit
ATTTACAGTCGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTA10.6711409395973155No Hit
ACACATAACTGGATTCTAAGACAGTCAACTGGAATTCAGAACACAGTTCC10.6711409395973155No Hit
GGCATGAGCCATCGTGCCCAGCCTCTGTTGATATTTTTCAAATAGAAAAT10.6711409395973155No Hit
AGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGA10.6711409395973155No Hit
GCAACATCCTTTCAGCTCTGCTGCTTTTTTGTCTTGGTGGAGGAATAGCC10.6711409395973155No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC10.6711409395973155No Hit
NATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GAGCTGTACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC10.6711409395973155No Hit
TGGAGGCAACGCCTGCCCAGTGCCACCTGTTTAAAAGCCGCGGGACCCTC10.6711409395973155No Hit
GCCTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGG10.6711409395973155No Hit
TCATAAAACCACCCAGTTGGATCCAAGGCAAATTTGTCACCTACTTCAGG10.6711409395973155No Hit
ATCATAGGCCAGGCCTATGATTTCACACCTGTAATCCCAGCACTTTCGGA10.6711409395973155No Hit
GTATCAAACCTAAATTATTTGTTATACTACTACTTTTAGACCAAAGATCC10.6711409395973155No Hit
GTCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTA10.6711409395973155No Hit
CCCAAGAAAAAGATGGAGGCAAGTTAACACAAGATTTTTTTTTAAAGATA10.6711409395973155No Hit
CCCCACTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCT10.6711409395973155No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC10.6711409395973155No Hit
GCATTTACGCCTGTAAATGTATTCATTCTTAATTTATGTAAGGTTTTTTT10.6711409395973155No Hit
CTCCTACCTCAGCCTTTGGAGTAGCTGGGACTACAGGCCACACCAACACT10.6711409395973155No Hit
GTTGCATAACTGGCATAAATCCTACTTGATCATGGTGTATAATTCTCTTT10.6711409395973155No Hit
CCTCAGATCCATCCTTTAAGCTGCTACTAGAGTGACGGTTGTAAGTTGAT10.6711409395973155No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG10.6711409395973155No Hit
GTCTATTATGGACCCAAATCCGTGGAGTTTTGTCTAGAAAAAACTGTGTC10.6711409395973155No Hit
GTGCTTCCAGATCATAGTGTTCAGTGTCCTCTGAAGTAACTGCCTGTTGC10.6711409395973155No Hit
GACAGTGACAGCACACCCTCACAGGTGCTGGGGGTTGACAAATGTCAGGT10.6711409395973155No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA10.6711409395973155No Hit
TTCTTTATCTGCCTCTTCCTACACATCGGGCGAGGCCTATATTACGGATC10.6711409395973155No Hit
GAGATGGACATTGCCTCTTCATTGTATTTCTCATCAATTCATTATTTTTG10.6711409395973155No Hit
CTTTTTGTCTAGCTGTTTGATTTCATTTTTTAATATCGGATGCCGGCTCC10.6711409395973155No Hit
TTGCTAAATTATGCAGTTAATCCTATGGTGCTTTAATTTTCAGGCCTTCA10.6711409395973155No Hit
TGCTTGTAATCCCAGCACTTTGGGAGGCTGAGATGGAAGGATCACTTGAG10.6711409395973155No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC10.6711409395973155No Hit
TGTTTAGTATATCCAGTATGATTGGTAAATGGGAAATATAATTGATTCTG10.6711409395973155No Hit
TTAATGATCTTTTAATACAGGGTTATTCAATCTTTTAGCTTCCCTGGGCC10.6711409395973155No Hit
GAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTA10.6711409395973155No Hit
ACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACA10.6711409395973155No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10.6711409395973155Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATA10.6711409395973155No Hit
GTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAAATTTGCCC10.6711409395973155No Hit
GTCAACGATCCCTCCCTTACCATCAAATCAATTGGCCACCAATGGTACTG10.6711409395973155No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC10.6711409395973155No Hit
TAACAAAGGTGCGATCTTGGGCTAATTTATTTGACCTCTCTGGACCTCAA10.6711409395973155No Hit
GCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTA10.6711409395973155No Hit
TTATTCATTCAGAATGCAATTCTGGGCCACTCTACAACCAAGAGAAAACC10.6711409395973155No Hit
CTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTT10.6711409395973155No Hit
TTGCAGCATTGCCGCGCGGGTGTTGTTATCCGCCACCGGACGCCAGGTTA10.6711409395973155No Hit
GTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCACC10.6711409395973155No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA10.6711409395973155No Hit
GTTGTATGTTTTCTTTGAGTCATATTTTGCTACTTGGGGAAGCAGATAGA10.6711409395973155No Hit
TTATTGTAAAACCCTGGGACTTGATTCTGATTATCTTACAAGAACCAGAG10.6711409395973155No Hit
GGCATAGAGACAGAGACAGAAACAGAGACAGAGACGGAAAAATTGAGTCA10.6711409395973155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA10.6711409395973155No Hit
CAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTG10.6711409395973155No Hit
ACATTGAGGTAATGACTAAAGAAATTTTCAGAGTTATATAAAAGCTATAG10.6711409395973155No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6711409395973155No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGT10.6711409395973155No Hit
AACATAAGTTAAAATCATAGGCACTAGTTCATCAAATCCACATTTCTTCT10.6711409395973155No Hit
TTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCC10.6711409395973155No Hit
GACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATT10.6711409395973155No Hit
GTATTAATATGTACATTAATAAGGCTAGCTTATGTAACATAAATATGTTA10.6711409395973155No Hit
GGGGGAATCTCAACAGATTAGTGGAAATTATAAAAAAAATACAGTGGAAG10.6711409395973155No Hit
GTACATGGGGCTGGGTGCGGTGGCTCACACCTGTAATCCCAGCACTTTGG10.6711409395973155No Hit
GTCTATCTCATTTCTTAGGTCTTTTAGTAATTGTTCTATAAATTTGGGAG10.6711409395973155No Hit
TTTTGGAGAAGTTGCTGTTTGGGACAATAGCATCAGTTTATCAAGAAGTG10.6711409395973155No Hit
CCCAGATTCTCTGGCCTTTTTAATGAGCTATTGTTAAACCAACAGGCTAG10.6711409395973155No Hit
ACTATTGTGTGCACAAAAATCACTTGGAAAGAATTGTTAAGCAAGTATTT10.6711409395973155No Hit
TATTGTAACTTACTACTCCGGAAAAAAAGAACCATTTGGATACATAGGTA10.6711409395973155No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAACC10.6711409395973155No Hit
GAGTACATGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGATTTCCGG10.6711409395973155No Hit
CATATACTCAATATTCTTCCATCCTTAATTATACCATAAGACGCAAACTT10.6711409395973155No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC10.6711409395973155No Hit
ACATTTTTCTGTCTCACAGTCACAATATTTCACATTTACGAGCTCTAATC10.6711409395973155No Hit
AGGGTAGACTTCCCCAACAAGCTGCATCTCTCTACCACCCTACCTTCGTT10.6711409395973155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10.6711409395973155No Hit
TTCCAGTTTTATGTCAAATAAAGAAGCATGCAGGAACAAGCTTTTCATAA10.6711409395973155No Hit
TAAATGTACCCTCTAGAATTACCATCCCTGAGGTATAGAATGTTGTTTTA10.6711409395973155No Hit
GTACATGGGAGATGTGGAATATTTGTCATAAATAAATAATGAAAACCTAA10.6711409395973155No Hit
CATTAAGGCCACGAGATTTGTGTCAGGCTCATCTTTGGCAAGGCTCACAC10.6711409395973155No Hit
GGCCAAGGATAGGGTGAGGGATGTTTTTGTGTTGAGGACATAAAGCACTT10.6711409395973155No Hit
AGCTGGGACTACAGGCGCCCGCCACCGTGCCCGGCTAATTTTTTGTATTT10.6711409395973155No Hit
GATGAATTGATGATATTGAAATGAAATTGAAAGATGAGATGAAATGATGA10.6711409395973155No Hit
GGAGAAAGAAGGAGAAGATAAATCACAACCTAAATCAATCAGAGAACGAC10.6711409395973155No Hit
CCATCGTGCTGGTAGAAGAGTCTTTGCTGTTGCTCCCGCAAGCCGTGCTC10.6711409395973155No Hit
GCCTGGGCGACAGAGTGAGACTGCATCACAAGAAAAAAAATTTGCAAGGG10.6711409395973155No Hit
ACCCTCCTGCTTCGGCCTCCTGAGTGGCTGGGACTGTAGGCCGCTGCCCC10.6711409395973155No Hit
CACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATAC10.6711409395973155No Hit
GATAAAACCATAGTGACTGATAAGTTCTGACTATGGAAATGGGGTTAAGA10.6711409395973155No Hit
TCGATTATCTGATAACGAAACCCGCTATAGACAAATTACTGCAACAACGC10.6711409395973155No Hit
AGGTTATGATTTTGTATTTCGTCCCATATCTGCTAATGCCAAACTTGTGA10.6711409395973155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
TTCACATACACAGACATCAAAGTGAATAGTATCACTTCTGAACCTGAATA10.6711409395973155No Hit
CTCCATTCCCATCCCCATCCCCAGCACCACATTTTATAGATAAGAAAACG10.6711409395973155No Hit
CCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTG10.6711409395973155No Hit
TCCTAGCCCTAAGTCTGGCCTATGAGTGACTACAAAAAGGATTAGACTGA10.6711409395973155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTCTT10.6711409395973155No Hit
GGATGGAAAGTGGTAAATGAACCCATAGGATTGCCTAGTTCTTACACTTT10.6711409395973155No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GGCAACGACTGAAATTTTGTCTCAAAAAAAAAAAAAAAAAAAAAACAAAA10.6711409395973155No Hit
TCTCCATTTTCAGAGCTGCTTCTGGGTGTGCCCTTTTGAAGCACATATAA10.6711409395973155No Hit
GTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCA10.6711409395973155No Hit
CAGCTCTTCCAGGAAGCTTTCTTGGATTTCCCCATACTTAAGACAGATGT10.6711409395973155No Hit
GGGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATAT10.6711409395973155No Hit
ATCTAGGAAAGTCTAAACTTTGGACCTACCTCTTTTTTTGATACTCATTT10.6711409395973155No Hit
GCCTACCCCTTCCTTGTACTATCCCTATGAAGCATAATTATAACAAGCTC10.6711409395973155No Hit
GGAGAAATGCAAATCAAAACCACAATAAGACACCATTTCACATCTACTAG10.6711409395973155No Hit
CACTTGTAGTTTAAAATGTTCTCATTTATAAAATCTCAATTTGATATTGA10.6711409395973155No Hit
GTGTAATGGACTGAATGAGGGTAACTGGGATTTCCTTCACCTCGCTTATC10.6711409395973155No Hit
GCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCG10.6711409395973155No Hit
TTCCTTTCCGCGGCTGCCCCCTGCTCCGCTCCCGGCTGTCCCGCAGCTGC10.6711409395973155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)