FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001319496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32.013422818791946No Hit
CTAGAAAGCCTTTGTATATTGCAATTTTTCTGTTTGGGAAAATCTAAGGA10.6711409395973155No Hit
CTTTTAAGGTAATCTCTCTGTCTTAAGACATTTTCCAGTTTCATGACAAT10.6711409395973155No Hit
AGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCACCGTC10.6711409395973155No Hit
AAATAAAAGACAAATCTGCTCACAGTGAAAATCCTTTAGAGAAATACATG10.6711409395973155No Hit
GAACATTATCTGCATATGCCAAAAAATTTTAAGCAAATGAAAGCTACCAA10.6711409395973155No Hit
TCCGTCCCCTTTCGTTGTAGACATTAAAATCCAATGTACCGCATAGCGAG10.6711409395973155No Hit
GTACATGGGTCTAAGTATGGGGTTTCAGTTCTCTCATGGCCTCAGAATTA10.6711409395973155No Hit
CTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGTGGATCACGAGGTCAG10.6711409395973155No Hit
GAGTACATGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACAT10.6711409395973155No Hit
CTCTACTCCCACTAATAGCTTTTTGATGACTTCTAGCAAGCCTCGCTAAC10.6711409395973155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
TACGGGTTCTCAGCGCGCCCTCGCGGCAGCGCCTTTCCCCTCATAACTAC10.6711409395973155No Hit
GGCCTTGTCCAACAGGAAAATATCACAATCCTAAACATATGCACCTAACA10.6711409395973155No Hit
CTCCATCATTTTACATTCCTACCAGTAAGTAGTATATGAAGAGTCTCAAT10.6711409395973155No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGCTGGCGGCCTTT10.6711409395973155No Hit
CTCCCAGTCCTAGCTGCAGGCATCACTATACTACTCACAGACCGCACCCT10.6711409395973155No Hit
GTATATCTTCTTTTGAGAATTGTCTATTCATGTCCTTAGCCTACTTTTTG10.6711409395973155No Hit
CTCTAGAGGTGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCGTATTTC10.6711409395973155No Hit
CATATATACAGCTTTTACTATTTCCCACACATGATTGTAGGTGCTGGGAA10.6711409395973155No Hit
GCCCGGCCTTACTTTTTTTTAAATGCTGTTTTATGTTTACTCTAAAACCA10.6711409395973155No Hit
GTATCCCAGAAGAGTACATCCCGGACATGCCCTCCTTTCTGGCTTTTGTC10.6711409395973155No Hit
TTGCTATGTTGCCCACGCCGCTCTCAAACCCCAACACTCAACACCAAACT10.6711409395973155No Hit
ATTAAGGAGAGAAGGAAGAGAAGTAAGCCGGGGGCGTCTTTGATTGTGTA10.6711409395973155No Hit
GCTGTGGTCCCAGCTACTCAAGAGGCTGAGGTGGGAGGATAACCAGAGCC10.6711409395973155No Hit
ACTTATATTCCATAATACATCAGTATAATCACCATTTACATACCCTCCCA10.6711409395973155No Hit
GGTCCGGACTCTCCATCTTCTTCCATTGGCTATTCCTCCACCAAGACAAA10.6711409395973155No Hit
GTACAGTAGAAAACACTAGACCTGATGTCAGAAAACGGATTCGAGTCATT10.6711409395973155No Hit
CCTGTGGGACCATTCTTTTTCCATACACTCATCATCTAGGTGGTATCGGT10.6711409395973155No Hit
ATCGCCCAACGACAAACCTAAACGTCCGCATACACCCCACAATGCAGCGC10.6711409395973155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC10.6711409395973155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT10.6711409395973155No Hit
GAGTCCAGGAGCTGCCAAGACCGGCCTGGGCAACATGGCAAAACCCCATC10.6711409395973155No Hit
GTATCAACCCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10.6711409395973155No Hit
ACCGACGCTGACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCG10.6711409395973155No Hit
GTAAATTTGAGAGGAGAAACAAGGTAAATTAGAATAAGGAGGACACCGGT10.6711409395973155No Hit
CTTCGAGGCACTCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT10.6711409395973155No Hit
TACTGGTCGAACATCGAGAATATAAACCCTATTGTTGATATGGACTCTAT10.6711409395973155No Hit
TTACAGATGTGTTCCCAAGACCTAAAGGGAAGGTGGCTTGTCCAAAGACA10.6711409395973155No Hit
GTGGCGGCATTGGATTGCAGATAGTAAAAGGGCTACCGAAGCTGACTAAC10.6711409395973155No Hit
TAACCACGCAGCGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.6711409395973155No Hit
GCACACCGCAAACGCAACATAAACCCGAACACAGACGCGCGGGAGGAACC10.6711409395973155No Hit
AACTAGGACACTCAGAATGGGGAATTTCTCTTTCACCTTCGTCCCAAATG10.6711409395973155No Hit
GTATCTTTACTATAAAAGCTATTGTGTAAGCTAGTCATATTAAGTTGTTG10.6711409395973155No Hit
ACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGG10.6711409395973155No Hit
CACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGCTGGGTCAGC10.6711409395973155No Hit
GTACCTTTCCATAAGGCTTTGGATGCTTGGTTTTAAGGCTGTTCCTCTGA10.6711409395973155No Hit
GAGTCCATGCAGGCCATGCATTGTAGCCGACAGACAGTAGGAATAGAGGA10.6711409395973155No Hit
GGTCGGCACTGTCATTGCGGCGCCTTTATTGAAGCCCCCTGGCAACACTT10.6711409395973155No Hit
GATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGG10.6711409395973155No Hit
GGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGAACGATCG10.6711409395973155No Hit
CCCAAGAACAGGGTTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT10.6711409395973155No Hit
GCCCCTGCGCCGACCATATGTAGTATCAGCCTAATGGGGGTGGCCACGTT10.6711409395973155No Hit
GGCATATGCTATGTGATCACAACCACAACCATTTTTTTTTTATTTTTGAG10.6711409395973155No Hit
CCTTCAATTCAGCGCATATATAACAGACAACCGACCACACTTCTAGGCAA10.6711409395973155No Hit
CTGCAAATACTTCTGCCTTCTTACAGGAACTGCAAGAAGACACTTCTGTC10.6711409395973155No Hit
GTCTCACTCTGTCACCCAGGCTGAAGTGCAGTGGCATGATCATAGCTCAT10.6711409395973155No Hit
TACCTAAATTTTATTTAAGATCTACTCACCCCATAGTTGCTAATTAATAA10.6711409395973155No Hit
ATCTGCAAGCGTGTAAGATCGTCGTGATAGCCACAGAAGCAGATCTTGGT10.6711409395973155No Hit
GTATCAACGCAGTACATGGGAGTCCGCAAGTCTCCGTGCGTTTGCTTTTA10.6711409395973155No Hit
GCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTT10.6711409395973155No Hit
TGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGAC10.6711409395973155No Hit
TAACTGTAAAGTTATCTTGAGATGTGGTACAATTTCAATCTGAAAGAGAA10.6711409395973155No Hit
GAGTACATGGTAAAAAATGTTTTTTAAGCATCATCCTCATTAAAGGAAGG10.6711409395973155No Hit
CCTGTAATCCCAGCGCTTTGGGAGGGCTGAGGCAGGCAGATCACCTGGGG10.6711409395973155No Hit
CATTCACACACACCACTGGATTTAGCTCCCTACATTAATTCTGCTATGTT10.6711409395973155No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155Clontech SMART CDS Primer II A (100% over 21bp)
CAACAGGGCACTGTGTTTTACCATAGAGCAGTCCCCCATATTCTTCCGGT10.6711409395973155No Hit
NNNANNNNGTTTNCGANCTCNNNNNNGGNNCNNNNNNNCNNNNTNNNNNA10.6711409395973155No Hit
CAACCCGGGGCTTACCACCCTCCCTCGCAAACTAACCTCCAAACTTCTTC10.6711409395973155No Hit
GTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGG10.6711409395973155No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA10.6711409395973155No Hit
TTCTTAGCTTTTCACAGTCTACTTAGAATTAACATTGTTTTTCTTCATGG10.6711409395973155No Hit
GGAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCT10.6711409395973155No Hit
TAAGGAATCAGATGTGGCGCCCTCTGCCTGACCAAATTCCATTAATTGTA10.6711409395973155No Hit
GGACATGGAAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG10.6711409395973155No Hit
GTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTT10.6711409395973155No Hit
CAGGTCATCCCGCAAATCATAAAATGGCACTTTTTGAAGTTTTATATCCG10.6711409395973155No Hit
ATCAGATATTTCTCATTCATCCAAATAAGTAAATTCTGCAAGTTTATGCC10.6711409395973155No Hit
ACTGAAGGGCCTATAACCACTCTTGCGTAAACCAAACTGTTGAAGCAACA10.6711409395973155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
ACCGCATACTTGTTTGGAACAGAATCTGATACAGAGAGCAAACTCAAATG10.6711409395973155No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6711409395973155No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA10.6711409395973155No Hit
ATACTGAAATACTGTGGTTTATTAAATTCAATTGCTATGTTATGTAACTC10.6711409395973155No Hit
GGAAAAGGCTGTTGAATGAATTTCTTTTTTTCTTCCAGGAATTTATGCTT10.6711409395973155No Hit
GAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATA10.6711409395973155No Hit
ACCTTGCATTCCTCTGAACAATGTGGATGGGAATTCCCCACTCCCTGGGT10.6711409395973155No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10.6711409395973155Clontech Universal Primer Mix Long (96% over 25bp)
TTCTAGAACTGGGGCAGGGAAAATACAAGCATCTATAGCCTGGGGCATTT10.6711409395973155No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCA10.6711409395973155No Hit
GTCTTGAACTTTACTGACCCTTTCTTTTGCCATCTTTTATCTGCTGTTAA10.6711409395973155No Hit
GGTGTGGGCCTTGCAAAATGATCAAGCCTTTCTTTCATGTGAGTATTAAA10.6711409395973155No Hit
TAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTC10.6711409395973155No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT10.6711409395973155No Hit
GTTTATGTTACAAGACAATATTTAGAACTTTAAATGAAAGGCTTTAAGAG10.6711409395973155No Hit
GACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAAT10.6711409395973155No Hit
GGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAA10.6711409395973155No Hit
TACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTG10.6711409395973155No Hit
AGTTAGGTGTTACCATAAGTATTAACAGAAGAAAAAGGGAAAGCACAAAC10.6711409395973155No Hit
GAAATATCATTCGGGCTTGATGTGGGGAGGGGTGTTTAAGGGGTTGGCTA10.6711409395973155No Hit
ATCGCGCAACGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGC10.6711409395973155No Hit
ACACCCAATTGCTCATTTTAAAATCTGGCTTTCCACGCCTGTAATTCCAG10.6711409395973155No Hit
CTTACATGGTATGTTTTGTTGTTAAAGTTTGCTAACTGAATAGGTCTAAC10.6711409395973155No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT10.6711409395973155No Hit
GACTATAGATGCGCGCCTCCATGCGCAGCTAATTTTTGTATTTTTAGTAG10.6711409395973155No Hit
GTTGTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGT10.6711409395973155No Hit
GAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGGT10.6711409395973155No Hit
ACTGCATTCCAGCCTGGGTGACAGAGAGAGACCCTATCTCAAAAATAAAA10.6711409395973155No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT10.6711409395973155No Hit
GTCACGAACTTTCTAGAGCGGACAGGTACGGGGTCGGTCATGAGGGGCAG10.6711409395973155No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC10.6711409395973155No Hit
GTACAGAAGTGAGTAGTCTATCCTGGTTCCAGCGAGGGGAAGATGTTTCA10.6711409395973155No Hit
GCATCGCATCACCAAACGCCATGCAATAAAACAACGATAACCCCAAATGT10.6711409395973155No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAACGCTCCATAGGGT10.6711409395973155No Hit
ACGCAGAGTACATGGGATTGTTTTAAAGTATTTATAAGAAATGGAAGCCT10.6711409395973155No Hit
GTGGTATCAACGCAGAGTACATGTTTACAAAGAGGTTTTATGAAAAGAAT10.6711409395973155No Hit
CACTTGGCAATTAAACCACATAAAAAGAGGACAAGACCCCCATCCTACAT10.6711409395973155No Hit
CGCTCCCGACCAACCGATGCTACCAGGCAGGCGTGTCTACTCGCCGAGCT10.6711409395973155No Hit
GAATCAGACCGATTTTGTGAAGGTGAAAAAATTTGCGGGCTACTGGCAGC10.6711409395973155No Hit
TCACCATGCTGGCCAGGCTGGTCTCGAACTCCTGACCTCATGATCTGCCC10.6711409395973155No Hit
AAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGC10.6711409395973155No Hit
GGTCAATGCTGCGCATTTACTACAGCCAAACGGCAACAGTTAGCGGAAAC10.6711409395973155No Hit
CTTGTACACATCTGACGCTGCCGACGAGTAGAGTGCGATGTCTAGTGATG10.6711409395973155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT10.6711409395973155No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC10.6711409395973155No Hit
TCTCCAGGGCGCTCGCCTGGTCCAATTGGGGGTGATGACATCAGTTATAT10.6711409395973155No Hit
CAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTC10.6711409395973155No Hit
TTTTTTATCATGACCTACAATGAGGCACCTTTATTTCATCTAGCCAAAAT10.6711409395973155No Hit
GTTTGTGGACAGACTGGGGGGGGGTTTTGGGGGTTTTTTTTTTTTTTTTA10.6711409395973155No Hit
GGTGAAGACGGTCATGACCACGGTAATGAGTTAATTTATCTTGTCCCGTG10.6711409395973155No Hit
GGTCAGCACCGTCAATGCTGCGCCTTTATGGCAGCCCACCGGCAGCAGTT10.6711409395973155No Hit
TTCTTTTTTTTTTTAATCACCGGTAAATGCGGGAGCTCCGCGCGCAGTTG10.6711409395973155No Hit
ATTATCCCATTTGTTCTCTATAATAATCTTATCAGCTGGGTAATGTTTTT10.6711409395973155No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG10.6711409395973155No Hit
TATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTAC10.6711409395973155No Hit
GAGCACTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCCGGGAAATGGGG10.6711409395973155No Hit
CGTATTCCCTGTGCTTATCACACAATCGAGAGAAGAGGGCAATTGCCACA10.6711409395973155No Hit
TCTTAACAGTCACAAGGTGAAAAGTCAAATGAAACAGTACAATTCTTGAT10.6711409395973155No Hit
CCCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTC10.6711409395973155No Hit
GTATCAAAGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA10.6711409395973155No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GTCTAATATCGCTCACCTCATCTTTTTACTTTTCATGTGACTCCTGGCTG10.6711409395973155No Hit
GCACCAGACAATTCTCCTTCCATTGTGGCCCAGGGAAGCTAAAAGATTGA10.6711409395973155No Hit
GGTCACAGTTTTGCTATAAAAACTTCTAGACAAGTTAAGTACAATAAAAG10.6711409395973155No Hit
ATAACAGACAGTATAGTTAGTCTTTCTTACTCTAAACACGTTTTGTCAAA10.6711409395973155No Hit
GATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGC10.6711409395973155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)