FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001319618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483424
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT265285.487522340636791Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT173773.594567088104852Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC108072.2355116833256106Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT105192.175936651883233Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT69751.442832792745085Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC54581.1290295889322832Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC54281.1228238564903688Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT51181.058697954590587Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC45120.9333421592639174Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG43160.8927980406434104Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA42530.8797660025153902Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA40330.8342572979413517Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC33160.6859402925795989Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC32480.6718739657112597Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25790.5334861322565698No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG23380.48363341497319123Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA22780.4712219500893625Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22660.46873965711259685Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22310.4614996359303634Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC21360.4418481498643013Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG20370.42136923280598393Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19630.4060617594492619No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT19550.40440689746475145Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT18820.38930628185609323Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG18230.3771016747203283Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC17640.3648970675845634Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT14970.3096660488515258Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14820.30656318263056864Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13880.28711855431257033Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG13120.27139736545972065Clontech Universal Primer Mix Long (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11880.24574700469980806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11460.23705897928112796No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10690.22113093268021447No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG10560.21844178195538494Clontech SMART CDS Primer II A (100% over 26bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG10480.2167869199708744Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA9900.2047891705831734Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9350.19341199443966373Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT9290.19217084795128087Clontech Universal Primer Mix Long (96% over 25bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT9290.19217084795128087Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA8660.17913880982326075Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7990.16527934070298536Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA7890.16321076322234726Clontech Universal Primer Mix Long (96% over 25bp)
AAAGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT7340.15183358707883762Clontech SMART CDS Primer II A (100% over 26bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA6930.14335241940822135Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6900.14273184616402992Clontech Universal Primer Mix Long (96% over 25bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG6880.1423181306679023Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6680.13818097570662607Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA6280.12990666578407362Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA5990.12390779109022307Clontech SMART CDS Primer II A (96% over 26bp)
GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC5960.12328721784603165Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC5500.11377176143509632Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5340.11046203746607533Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5330.1102551797180115Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT5140.1063248825047991Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.104256305024161Clontech SMART CDS Primer II A (100% over 21bp)
GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5020.10384258952803335Clontech Universal Primer Mix Long (100% over 24bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC4990.10322201628384194Clontech SMART CDS Primer II A (96% over 26bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC4980.10301515853577811Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4980.10301515853577811Clontech Universal Primer Mix Long (96% over 27bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT4900.10136029655126763Clontech Universal Primer Mix Long (96% over 28bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG4900.10136029655126763Clontech SMART CDS Primer II A (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)