FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001319630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176738
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT764093.510252497085088Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT520382.3906414093014408Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC326001.4976538288025476Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT284431.3066799954794743Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT203080.9329556428012926Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC167610.7700053933913957Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT151480.6959036870767176Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC139370.6402699819638377Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC124480.5718648730347888Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA116060.5331831391743058Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA113520.5215143025940651Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG111460.5120506004856809Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC103130.4737823293386709Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC98440.452236327936573Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC70020.3216739910820687Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG66970.30766219912548043Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA65540.3010927360114079Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60630.27853604797637566No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC57490.2641107933063143Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT55370.25437144938894807Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG55250.25382016577098393Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT53030.24362141883864757Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG45680.20985529723834473Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45390.2085230284949314No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA44400.20397493864672736Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA44280.20342365502876322Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG40880.1878039525197796Clontech Universal Primer Mix Long (96% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35990.1653391450877414No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35700.1640068763443281No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG35300.16216926428444767Clontech SMART CDS Primer II A (100% over 26bp)
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32370.14870875594582353Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31920.14664144237845805Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA31590.1451254124290567Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30540.14030168077187058Clontech Universal Primer Mix Long (96% over 26bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG30190.1386937702194752Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT29830.13703991936558282Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT28100.12909224720659998Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26040.11962854509821577No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT25930.11912320178174866Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23560.10823535032695714Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA21980.10097678269042944Clontech Universal Primer Mix Long (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)