FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001319908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2081057
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT96720.46476381953978196Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54420.26150172724725945Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41310.1985048943878039Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40500.1946126415566705No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37260.17904363023213685Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33340.16020704862961468No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31070.1492991302016235Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24810.11921826264249369Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24050.11556627233180061Clontech Universal Primer Mix Long (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)