FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001319972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001319972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361876
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT343209.483911616133703Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT210125.806408825122418Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT147294.070178735257381Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC138283.8211984215587664Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT102762.839646729819054Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT88882.4560899313577025Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC77542.1427229216637746Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC75022.073085808398457Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC65061.7978534083498217Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA60751.6787518376460444Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG58401.6138124661486255Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC52031.4377853187279621Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA51951.4355746167195391Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC46711.2907736351678476Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC36871.0188572881318463Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA35660.9854204202544518Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT32290.8922945981496424Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG31890.8812410881075285Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG31570.8723982800738375Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC30230.8353690214327559Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT27010.7463882655937393Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG21000.5803092772109784Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18430.5092904751903967Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16110.44518011694613624No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT15720.43440294465507523Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT15360.42445478561717276Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14970.41367761332611175Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG14040.3879782024781969Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA13510.37333230167239606Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12000.3316053012634162No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA10810.2987211088881274Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT10610.2931943538670705Clontech SMART CDS Primer II A (96% over 26bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG10510.290430976356542Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG9810.2710873337828428Clontech SMART CDS Primer II A (96% over 26bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA9730.26887663177442Clontech Universal Primer Mix Long (96% over 25bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA9480.2619681879980988Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC8080.22328090285070024Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT7640.211122041804375Clontech Universal Primer Mix Long (96% over 28bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC6870.18984403497330576Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6240.1724347566569764No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT6050.16718433938697233Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA5940.16414462412539102Clontech SMART CDS Primer II A (96% over 26bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG5920.16359194862328533Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5670.15668350484696417Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT5450.15060407432380152Clontech SMART CDS Primer II A (96% over 26bp)
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC5100.1409322530369519Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5060.1398269020327405Clontech Universal Primer Mix Long (96% over 26bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT4690.12960240524378516Clontech Universal Primer Mix Long (96% over 28bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC4610.1273917032353624Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4570.126286352231151Clontech SMART CDS Primer II A (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCCTGTC4560.12601001448009816Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC4550.1257336767290453Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG4520.12490466347588676Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4390.12131227271219977Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4240.11716720644640705Clontech Universal Primer Mix Long (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3970.10970608716798019No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC3930.10860073616376881Clontech SMART CDS Primer II A (96% over 26bp)
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG3760.1039029943958704Clontech Universal Primer Mix Long (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)